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A New Peptide-based Chemosensor for Selective Imaging of Copper ion and Hydrogen Sulfide in Living Cells
Microchemical Journal ( IF 4.8 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.microc.2020.104658
Chaowei Hao , Yongxin Li , Baomin Fan , Guangzhi Zeng , Danni Zhang , Zhaoxiang Bian , Jiang Wu

Abstract Hydrogen sulfide (H2S), like CO and NO, is an endogenous gas signal molecule that can play a vital role in many physiological processes. Therefore, it's of great significance to study the efficient and sensitive imaging technology of H2S in vivo. Copper is an essential trace element in the human body that is very important for many cellular functions and related metabolic processes. In this study we report the design of a peptide L (FITC-Ahx-His-Glu-Phe-His-NH2) that can act as an “On-Off-On” fluorescent chemical sensor to detect Cu2+ and S2−, and cysteine (Cys) and glutathione (GSH) can also replace Cu2+ from L-Cu system. The capability of this peptide fluorescent probe to detect H2S in HeLa cells is also evaluated.

中文翻译:

一种用于活细胞中铜离子和硫化氢选择性成像的新型肽基化学传感器

摘要 硫化氢(H2S)与 CO 和 NO 一样,是一种内源性气体信号分子,在许多生理过程中发挥着重要作用。因此,研究高效、灵敏的体内硫化氢成像技术具有重要意义。铜是人体必需的微量元素,对许多细胞功能和相关代谢过程非常重要。在这项研究中,我们报告了一种肽 L (FITC-Ahx-His-Glu-Phe-His-NH2) 的设计,它可以作为“开-关-开”荧光化学传感器来检测 Cu2+ 和 S2-,以及半胱氨酸(Cys) 和谷胱甘肽 (GSH) 也可以替代 L-Cu 系统中的 Cu2+。还评估了该肽荧光探针检测 HeLa 细胞中 H2S 的能力。
更新日期:2020-05-01
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