A novel, ex-situ remediation process for perchlorate contaminated soil is reported in this study. This approach comprises washing the contaminated soil with water, followed by treatment of the wash water in a bioreactor. The treated water reused for the next batch of soil, and the cycle continued. The pilot-scale treatment unit comprising of a soil washing unit (0.75 m3) and a fixed-film bioreactor (140 L), both connected in series for continuous operation for a period of three months. The bioreactor was inoculated with a novel perchlorate reducing microbial consortium comprising Serratia marcescens (Gen bank no. HM751096), Bacillus pumilus (Gen bank no. JQ820452) and Micrococcus sp. (Gen bank no. KJ410671). The microbial activity was supported by glucose (glucose/perchlorate ratio = 5), and trace mineral solution. In a typical washing cycle, 2.5 g perchlorate (KClO4) spiked in 670 kg soil was completely removed in three washing cycles, that completed in 6.3 h consuming ∼360 L water. The pooled wash water containing perchlorate at 8.5 mg/L was treated completely in the bioreactor operated at 4.5 h HRT and -200 mV ORP. Compared with both in-situ and ex-situ remediation methods reported, the present approach has many advantages for treating perchlorate contaminated soil.

中文翻译：

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一种高氯酸盐污染土壤的新型异位生物修复方法。

这项研究报道了一种新颖的非原位修复高氯酸盐污染土壤的方法。该方法包括用水洗涤被污染的土壤，然后在生物反应器中处理洗涤水。经过处理的水可用于下一批土壤，并且循环继续进行。中试规模的处理单元由一个土壤洗涤单元（0.75 m3）和一个固定膜生物反应器（140 L）组成，两个单元串联连接，可连续运行三个月。该生物反应器用包括高粘质沙雷氏菌（Serratia marcescens）（Gen库号HM751096），短小芽孢杆菌（Bacillus pumilus）（Gen库号JQ820452）和Micrococcus sp。的新型高氯酸盐还原微生物菌群接种。（Gen bank No.KJ410671）。葡萄糖（葡萄糖/高氯酸盐之比= 5）和微量矿物质溶液支持微生物的活性。在典型的洗涤周期中，2。在三个洗涤周期中，完全去除了掺入670 kg土壤中的5 g高氯酸盐（KClO4），该过程在6.3小时内完成，消耗约360 L水。在4.5 h HRT和-200 mV ORP下运行的生物反应器中，将含有8.5 mg / L高氯酸盐的合并洗涤水完全处理。与报道的原位和异位修复方法相比，本方法具有处理高氯酸盐污染土壤的许多优点。