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Stable Isotopes for Tracing Mammalian-Cell Metabolism In Vivo.
Trends in Biochemical Sciences ( IF 13.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.tibs.2019.12.002 Juan Fernández-García 1 , Patricia Altea-Manzano 1 , Erica Pranzini 2 , Sarah-Maria Fendt 1
Trends in Biochemical Sciences ( IF 13.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.tibs.2019.12.002 Juan Fernández-García 1 , Patricia Altea-Manzano 1 , Erica Pranzini 2 , Sarah-Maria Fendt 1
Affiliation
Metabolism is at the cornerstone of all cellular functions and mounting evidence of its deregulation in different diseases emphasizes the importance of a comprehensive understanding of metabolic regulation at the whole-organism level. Stable-isotope measurements are a powerful tool for probing cellular metabolism and, as a result, are increasingly used to study metabolism in in vivo settings. The additional complexity of in vivo metabolic measurements requires paying special attention to experimental design and data interpretation. Here, we review recent work where in vivo stable-isotope measurements have been used to address relevant biological questions within an in vivo context, summarize different experimental and data interpretation approaches and their limitations, and discuss future opportunities in the field.
中文翻译:
用于体内追踪哺乳动物细胞代谢的稳定同位素。
代谢是所有细胞功能的基石,越来越多的证据表明其在不同疾病中的失调强调了在全生物水平上全面理解代谢调节的重要性。稳定同位素测量是探测细胞代谢的有力工具,因此越来越多地用于体内条件下的代谢研究。体内代谢测量的额外复杂性需要特别注意实验设计和数据解释。在这里,我们回顾了最近的工作,其中体内稳定同位素测量已用于解决体内背景下的相关生物学问题,总结了不同的实验和数据解释方法及其局限性,并讨论了该领域的未来机会。
更新日期:2020-01-17
中文翻译:
用于体内追踪哺乳动物细胞代谢的稳定同位素。
代谢是所有细胞功能的基石,越来越多的证据表明其在不同疾病中的失调强调了在全生物水平上全面理解代谢调节的重要性。稳定同位素测量是探测细胞代谢的有力工具,因此越来越多地用于体内条件下的代谢研究。体内代谢测量的额外复杂性需要特别注意实验设计和数据解释。在这里,我们回顾了最近的工作,其中体内稳定同位素测量已用于解决体内背景下的相关生物学问题,总结了不同的实验和数据解释方法及其局限性,并讨论了该领域的未来机会。