Reporter gene imaging (RGI) is described as the methodology that involves imaging of the encoding proteins that can be used as surrogate markers when fused with regulatory regions of the gene of interest. It provides a means to indirectly monitor molecular processes that are implicated in the pathophysiology of several diseases. The modalities utilized in RGI include MRI, PET, SPECT, as well as optical imaging modalities, such as bioluminescence and fluorescence. RGI provides a highly specific way to qualitatively and quantitatively assess cell targeting, transfection, protein expression and other intracellular processes, which are valuable for pharmacodynamic and pharmacokinetic assessment of cellular, gene and oncolytic viral therapeutics.

中文翻译：

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报告基因成像及其在基于成像的药物开发中的作用。

报告基因成像 (RGI) 被描述为涉及编码蛋白质成像的方法，当与感兴趣的基因的调控区域融合时，可以用作替代标记。它提供了一种间接监测与多种疾病的病理生理学有关的分子过程的方法。RGI 中使用的模式包括 MRI、PET、SPECT 以及光学成像模式，例如生物发光和荧光。RGI 提供了一种高度特异性的方法来定性和定量评估细胞靶向、转染、蛋白质表达和其他细胞内过程，这对于细胞、基因和溶瘤病毒疗法的药效学和药代动力学评估很有价值。