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Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time.
Molecular Cell ( IF 16.0 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.molcel.2019.12.018
Brandon J Davis 1 , Mitchell R O'Connell 1
Affiliation  

Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 approach to image colocalized or repeat-containing RNAs in real time, as well as demonstrate simultaneous RNA-DNA labeling by using Cas13 and Cas9 in tandem.

中文翻译:

戴上你的副眼镜:开发基于 CRISPR-Cas13 的优化实时图像 RNA 动力学方法。

活细胞 RNA 成像是一种观察 RNA 代谢实时动态的强大方法。最近的两篇论文描述了一种优化的基于荧光的 CRISPR-Cas13 方法,用于对共定位或包含重复的 RNA 进行实时成像,并展示了通过串联使用 Cas13 和 Cas9 进行同步 RNA-DNA 标记。
更新日期:2020-01-16
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