Abstract Duhaldea nervosa (Wallich ex Candolle) A. Anderberg was widely used for food spice and folk medicine. However, it is still insufficient in the constituent’s characterization of D. nervosa. In this study, a systematic strategy for rapid detection and identification of constituents was proposed based on UHPLC-Q-Exactive Orbitrap mass spectrometry in parallel reaction monitoring mode combining anion exchange resin separation, expected compounds predicted and diagnosis fragmentation ions techniques. Finally, 149 chlorogenic acids derivatives were unanimously and tentatively characterized from D. nervosa, 102 of them were report for the first time. This results widely extended the chemical constituents of D. nervosa, which will facilitate understanding the effective substance and quality control. Meantime, it is possible for this strategy to exhibit a wide application for chemical’s characterization in different sample.

中文翻译：

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使用 UHPLC-Q-Exactive Orbitrap 质谱法快速鉴定 Duhaldea nervosa 中绿原酸衍生物的系统策略

摘要 Duhaldea nervosa (Wallich ex Candolle) A. Anderberg 被广泛用于食用香料和民间药物。然而，它仍然不足以表征 D. nervosa 的成分。在本研究中，基于 UHPLC-Q-Exactive Orbitrap 质谱在平行反应监测模式下结合阴离子交换树脂分离、预期化合物预测和诊断碎裂离子技术，提出了一种快速检测和鉴定成分的系统策略。最后，149 种绿原酸衍生物在 D. nervosa 中得到一致初步鉴定，其中 102 种为首次报道。这一结果广泛扩展了 D. nervosa 的化学成分，这将有助于了解有效物质和质量控制。与此同时，