The development of sensitive methods for in situ detection of biomarkers is a real challenge to bring medical diagnosis a step forward. The proof-of-concept of a remote multiplexed biomolecular interaction detection through a plasmonic optical fiber bundle is demonstrated here. The strategy relies on a fiber optic biosensor designed from a 300 µm diameter bundle composed of 6000 individual optical fibers. When appropriately etched and metallized, each optical fiber exhibits specific plasmonic properties. The surface plasmon resonance phenomenon occurring at the surface of each fiber enables to measure biomolecular interactions, through the changes of the retro-reflected light intensity due to light/plasmon coupling variations. The functionalization of the microstructured bundle by multiple protein probes was performed using new polymeric 3D-printed microcantilevers. Such soft cantilevers allow for immobilizing the probes in micro spots, without damaging the optical microstructures nor the gold layer. We show here the potential of this device to perform the multiplexed detection of two different antibodies with limits of detection down to a few tenths of nanomoles per liter. This tool, adapted for multiparametric, real-time, and label free monitoring is minimally invasive and could then provide a useful platform for in vivo targeted molecular analysis.

中文翻译：

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通过光纤束对生物相互作用进行多路远程SPR检测。

开发用于生物标志物原位检测的灵敏方法是使医学诊断向前迈进的真正挑战。这里展示了通过等离激元光纤束进行的远程多路复用生物分子相互作用检测的概念验证。该策略依赖于光纤生物传感器，该传感器由直径300 µm的光纤束设计而成，该光纤束由6000条单根光纤组成。经过适当的蚀刻和金属化处理后，每条光纤都会表现出特定的等离子体特性。发生在每根纤维表面的表面等离振子共振现象能够通过由于光/等离激元耦合变化而引起的回射光强度的变化来测量生物分子的相互作用。使用新型聚合物3D打印的微悬臂梁，通过多个蛋白质探针对微结构束进行功能化。这种柔软的悬臂允许将探针固定在微点上，而不会损坏光学微结构或金层。我们在这里展示了该设备执行两种不同抗体的多重检测的潜力，其检测极限低至每升十分之几纳摩尔。该工具适用于多参数，实时和无标签监控，具有微创性，可为体内靶向分子分析提供有用的平台。我们在这里展示了该设备执行两种不同抗体的多重检测的潜力，其检测极限低至每升十分之几纳摩尔。该工具适用于多参数，实时和无标签监控，具有微创性，可为体内靶向分子分析提供有用的平台。我们在这里展示了该设备执行两种不同抗体的多重检测的潜力，其检测限低至每升十分之几纳摩尔。该工具适用于多参数，实时和无标签监控，具有微创性，可为体内靶向分子分析提供有用的平台。