A method is described for the simultaneous determination of hepatocellular carcinoma-associated microRNA-122 and microRNA-199a/b-3p. This probe consists of two kinds of nanomaterials. The first comprises CdTe@CdS core-shell quantum dots which, on excitation at 375 nm give two emissions, with peak wavelengths at 543 (g-QDs) and at 627 nm (r-QDs). The second comprises gold nanoparticles acting as a quencher. In the absence of the target, g-QD-N1 and r-QD-N2 are stable due to the fluorescence stability. With the addition of microRNA-122 and microRNA-199a/b-3p, g-QD-N1 and r-QD-N2 are conjugated to the surface of AuNP-S1/S2 through base complementary pairing. As a result, fluorescence resonance energy transfer (FRET) occurs, resulting in a decrease at 550 nm and 635 nm respectively, which can realize the simultaneous detection of two different microRNAs. Detection is achieved within 50 min. The detection limits (3σ/k) are 0.2 nM for microRNA-122 and 0.5 nM for microRNA-199a/b-3p. The clinical applicability of the assay was demonstrated by detecting microRNAs in human serum and different cell lysates. Graphical abstract Schematic for the simultaneous determination of microRNA-122 and microRNA-199a/b-3p by FRET.

中文翻译：

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单激发双发射 CdTe@CdS 量子点用于多种肿瘤相关 microRNA 的荧光杂交测定

描述了一种同时测定肝细胞癌相关 microRNA-122 和 microRNA-199a/b-3p 的方法。该探针由两种纳米材料组成。第一个包括 CdTe@CdS 核壳量子点，在 375 nm 激发时产生两种发射，峰值波长分别为 543 (g-QDs) 和 627 nm (r-QDs)。第二种包含充当猝灭剂的金纳米颗粒。在没有目标的情况下，由于荧光稳定性，g-QD-N1 和 r-QD-N2 是稳定的。通过添加 microRNA-122 和 microRNA-199a/b-3p，g-QD-N1 和 r-QD-N2 通过碱基互补配对结合到 AuNP-S1/S2 的表面。结果，发生荧光共振能量转移（FRET），导致分别在 550 nm 和 635 nm 处减少，可实现两种不同microRNA的同时检测。检测在 50 分钟内完成。microRNA-122 的检测限 (3σ/k) 为 0.2 nM，microRNA-199a/b-3p 的检测限为 0.5 nM。通过检测人血清和不同细胞裂解物中的微小 RNA 证明了该测定的临床适用性。通过 FRET 同时测定 microRNA-122 和 microRNA-199a/b-3p 的图形摘要示意图。