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A network composed of gold nanoparticles and a poly(vinyl alcohol) hydrogel for colorimetric determination of ceftriaxone
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-01-15 , DOI: 10.1007/s00604-019-4039-8 Nahid Pourreza 1 , Matineh Ghomi 1
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-01-15 , DOI: 10.1007/s00604-019-4039-8 Nahid Pourreza 1 , Matineh Ghomi 1
Affiliation
A hydrogel network was prepared from poly(vinyl alcohol) (PVA) and borax, and then was modified with gold nanoparticles (AuNPs) that were obtained by in-situ nucleation and growth. This modified network is shown to be a viable optical nanoprobe for the drug ceftriaxone (CTRX) in biological samples. The properties and morphology of the modified network were investigated using energy dispersive X-ray analysis, transmission electron microscopy, zeta-sizing and viscosimetry. The UV-vis spectrum was recorded to verify the nanosynthesis of the red AuNPs, and the maximum absorption is found at 517 nm. This AuNP-poly(vinyl alcohol)-borax hydrogel nanoprobe (AuNP/PBH) is introduced as an optical nanoprobe for ceftriaxone in biological samples. The AuNPs have a better ability to attach the sulfur functional groups than amino functional groups. Hence, the probable mechanism is based on the attachment of sulfur functional groups of CRTX structure with AuNPs located in the PBH. As a result of this interaction, the surface plasmon resonance of AuNPs is altered in the presence of CTRX and the absorption of the nanoprobe is decreased at 517 nm. The effects of pH value, borax and PVA concentration were investigated. Under optimum conditions, the calibration graph is linear in the 1–90 μg mL −1 CTRX concentration range, and the limit of detection is 0.33 μg mL −1 . The relative standard deviation for ten replicate measurements of at levels of 20 and 70 μg mL −1 of CTRX was 4.0% and 2.2%, respectively. The nanoprobe was successfully applied to the determination of CTRX in (spiked) serum and urine samples. The performance of the nanoprobe was compared with HPLC method and the results were satisfactory. Graphical abstract Schematic representation of a new nanoprobe based on in situ formation of AuNPs into poly(vinyl alcohol) (PVA)-borax (PBH) hydrogel fabricated for ceftriaxone detection. The hydrogel acts as the reducing agent for production and embedding of AuNPs in the network.
中文翻译:
由金纳米粒子和聚乙烯醇水凝胶组成的网络,用于比色测定头孢曲松
由聚乙烯醇 (PVA) 和硼砂制备水凝胶网络,然后用通过原位成核和生长获得的金纳米粒子 (AuNPs) 进行改性。这种改进的网络被证明是生物样品中药物头孢曲松 (CTRX) 的可行光学纳米探针。使用能量色散 X 射线分析、透射电子显微镜、zeta 尺寸和粘度测定法研究了改性网络的性质和形态。记录 UV-vis 光谱以验证红色 AuNP 的纳米合成,并且在 517 nm 处发现最大吸收。这种 AuNP-聚(乙烯醇)-硼砂水凝胶纳米探针 (AuNP/PBH) 被引入作为生物样品中头孢曲松的光学纳米探针。与氨基官能团相比,AuNPs 具有更好的附着硫官能团的能力。因此,可能的机制是基于 CRTX 结构的硫官能团与位于 PBH 中的 AuNP 的连接。由于这种相互作用,AuNPs 的表面等离子体共振在 CTRX 存在下发生改变,并且纳米探针的吸收在 517 nm 处降低。研究了pH值、硼砂和PVA浓度的影响。在最佳条件下,校准图在 1–90 μg mL -1 CTRX 浓度范围内呈线性,检测限为 0.33 μg mL -1 。在 20 和 70 μg mL -1 水平的 CTRX 的十次重复测量的相对标准偏差分别为 4.0% 和 2.2%。纳米探针成功应用于测定(加标)血清和尿液样品中的 CTRX。将纳米探针的性能与HPLC法进行了比较,结果令人满意。图形摘要 基于 AuNPs 原位形成到聚(乙烯醇)(PVA)-硼砂(PBH)水凝胶中的新型纳米探针的示意图,该水凝胶用于头孢曲松检测。水凝胶充当还原剂,用于生产和嵌入网络中的 AuNP。
更新日期:2020-01-15
中文翻译:
由金纳米粒子和聚乙烯醇水凝胶组成的网络,用于比色测定头孢曲松
由聚乙烯醇 (PVA) 和硼砂制备水凝胶网络,然后用通过原位成核和生长获得的金纳米粒子 (AuNPs) 进行改性。这种改进的网络被证明是生物样品中药物头孢曲松 (CTRX) 的可行光学纳米探针。使用能量色散 X 射线分析、透射电子显微镜、zeta 尺寸和粘度测定法研究了改性网络的性质和形态。记录 UV-vis 光谱以验证红色 AuNP 的纳米合成,并且在 517 nm 处发现最大吸收。这种 AuNP-聚(乙烯醇)-硼砂水凝胶纳米探针 (AuNP/PBH) 被引入作为生物样品中头孢曲松的光学纳米探针。与氨基官能团相比,AuNPs 具有更好的附着硫官能团的能力。因此,可能的机制是基于 CRTX 结构的硫官能团与位于 PBH 中的 AuNP 的连接。由于这种相互作用,AuNPs 的表面等离子体共振在 CTRX 存在下发生改变,并且纳米探针的吸收在 517 nm 处降低。研究了pH值、硼砂和PVA浓度的影响。在最佳条件下,校准图在 1–90 μg mL -1 CTRX 浓度范围内呈线性,检测限为 0.33 μg mL -1 。在 20 和 70 μg mL -1 水平的 CTRX 的十次重复测量的相对标准偏差分别为 4.0% 和 2.2%。纳米探针成功应用于测定(加标)血清和尿液样品中的 CTRX。将纳米探针的性能与HPLC法进行了比较,结果令人满意。图形摘要 基于 AuNPs 原位形成到聚(乙烯醇)(PVA)-硼砂(PBH)水凝胶中的新型纳米探针的示意图,该水凝胶用于头孢曲松检测。水凝胶充当还原剂,用于生产和嵌入网络中的 AuNP。