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Optimized Nanointerface Engineering of Micro/Nanostructured Titanium Implants to Enhance Cell–Nanotopography Interactions and Osseointegration
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2020-01-23 , DOI: 10.1021/acsbiomaterials.9b01717
Kai Li 1 , Shiwei Liu 1, 2 , Tao Hu 3 , Ihar Razanau 4 , Xiaodong Wu 5 , Haiyong Ao 6 , Liping Huang 1 , Youtao Xie 1 , Xuebin Zheng 1
Affiliation  

The success of orthopedic implants requires rapid and complete osseointegration which relies on an implant surface with optimal features. To enhance cellular function in response to the implant surface, micro- and nanoscale topography have been suggested as essential. The aim of this study was to identify an optimized Ti nanostructure and to introduce it onto a titanium plasma-sprayed titanium implant (denoted NTPS-Ti) to confer enhanced immunomodulatory properties for optimal osseointegration. To this end, three types of titania nanostructures, namely, nanowires, nanonests, and nanoflakes, were achieved on hydrothermally prepared Ti substrates. The nanowire surface modulated protein conformation and directed integrin binding and specificity in such a way as to augment the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and induce a desirable osteoimmune response of RAW264.7 macrophages. In a coculture system, BMSCs on the optimized micro/nanosurface exerted enhanced effects on nonactivated or lipopolysaccharide-stimulated macrophages, causing them to adopt a less inflammatory macrophage profile. The enhanced immunomodulatory properties of BMSCs grown on NTPS-Ti depended on a ROCK-medicated cyclooxygenase-2 (COX2) pathway to increase prostaglandin E2 (PGE2) production, as evidenced by decreased production of PGE2 and concurrent inhibition of immunomodulatory properties after treatment with ROCK or COX2 inhibitors. In vivo evaluation showed that the NTPS-Ti implant resulted in enhanced osseointegration compared with the TPS-Ti and Ti implants. The results obtained in our study may provide a prospective approach for enhancing osseointegration and supporting the application of micro/nanostructured Ti implants.

中文翻译:

优化的微/纳米结构钛植入物纳米界面工程,以增强细胞-纳米形貌的相互作用和骨整合。

整形外科植入物的成功需要快速而完整的骨整合,这取决于具有最佳功能的植入物表面。为了增强响应于植入物表面的细胞功能,已建议微米和纳米级的形貌。这项研究的目的是确定优化的Ti纳米结构,并将其引入钛等离子喷涂的钛植入物(表示为NTPS-Ti),以赋予增强的免疫调节特性,以实现最佳的骨整合。为此,在水热制备的Ti衬底上获得了三种类型的二氧化钛纳米结构,即纳米线,纳米巢和纳米薄片。纳米线表面调节蛋白构象并指导整联蛋白结合和特异性,以增强骨髓来源的间充质干细胞(BMSCs)的成骨分化并诱导RAW264.7巨噬细胞产生理想的骨免疫应答。在共培养系统中,优化的微/纳米表面上的BMSC对未活化的或脂多糖刺激的巨噬细胞产生增强的作用,从而使它们采用较少的炎症性巨噬细胞谱。在NTPS-Ti上生长的BMSCs增强的免疫调节特性取决于ROCK药物加氧合酶2(COX2)途径来增加前列腺素E2(PGE2)的产生,这一点可通过PGE2的产生减少以及在用ROCK治疗后同时抑制免疫调节特性来证明或COX2抑制剂。体内评估显示,与TPS-Ti和Ti植入物相比,NTPS-Ti植入物增强了骨整合。在我们的研究中获得的结果可能为增强骨整合和支持微/纳米结构钛植入物的应用提供前瞻性方法。
更新日期:2020-01-23
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