Monitoring of the hemostasis status is essential for therapeutic anticoagulants, undergoing surgery, cardiovascular diseases, etc. Although the clinical values of conventional blood coagulation tests have been well demonstrated, these devices have limitations such as large and expensive equipment, excessive sample volumes, long turnaround times, and difficulty in miniaturization for point-of-care use. Here, we present a novel strategy to evaluate blood hemostasis using the single-port Love-mode surface acoustic wave (SLSAW) sensor. The SLSAW sensor was designed as a plug-and-play-type unit for disposable use and operated under the harmonic resonant mode to produce frequency response to the blood coagulation cascade. Compared with a quartz crystal microbalance, Lamb wave, and film bulk acoustic resonator, the frequency shift of SLSAW was significantly increased, ranging from approximately 8960 to 10 368 kHz, which indicated enhancement of the signal-to-noise ratio. To demonstrate the feasibility of the SLSAW, studies were carried out to examine the effects of temperature and clotting reagents on coagulation times and kinetics. Activated partial thromboplastin times of plasma were validated by comparing with SYSMEX CA-7000 with the correlation (R2) as 0.996. In terms of coagulation kinetics, reaction time, clot formation time, maximum frequency shift, and clot formation rate of whole blood correlated well with corresponding parameters of the standard thromboelastography (TEG) analyzer (R2 = 0.9942, 0.9868, 0.9712, and 0.9939, respectively). The SLSAW sensor, with the advantages of low cost, small size, little sample consumption (1 μL), disposable use, and simple operation, is a promising tool for point-of-care diagnosis of hemostasis.

中文翻译：

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使用Love-mode表面声波传感器对止血进行即时护理评估。

止血状态的监测对于治疗性抗凝剂，外科手术，心血管疾病等至关重要。尽管已经很好地证明了常规凝血试验的临床价值，但这些设备仍存在局限性，例如设备庞大且昂贵，样品量过多，周转时间长时间，并且难以在医疗点使用时实现小型化。在这里，我们提出了一种使用单端口洛夫模式表面声波（SLSAW）传感器评估血液止血的新颖策略。SLSAW传感器设计为即插即用型单元，可一次性使用，并在谐波谐振模式下运行，以产生对凝血级联的频率响应。与石英微天平，兰姆波和薄膜体声谐振器相比，SLSAW的频移显着增加，范围从大约8960到10 368 kHz，这表明信噪比有所提高。为了证明SLSAW的可行性，进行了研究以检查温度和凝结剂对凝血时间和动力学的影响。通过与SYSMEX CA-7000进行比较，验证血浆的活化部分凝血活酶时间，相关性（R2）为0.996。就凝血动力学而言，全血的反应时间，血凝块形成时间，最大频移和血凝块形成速率与标准血栓弹力图（TEG）分析仪的相应参数具有良好的相关性（R2分别为0.9942、0.9868、0.9712和0.9939） ）。SLSAW传感器具有成本低，体积小，样品消耗少（1μL），