Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under "serum-free" conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons.

中文翻译：

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合成聚合物为功能神经元培养提供了坚固的底物。

神经元培养和植入的底物必须具有生物相容性，并显示支持细胞附着，生长，分化和神经活动的表面成分。层粘连蛋白是一种天然存在的细胞外基质蛋白，是神经元培养中使用最广泛的底物，可以满足其中一些要求，但是，它价格昂贵，不稳定（与合成材料相比），并且易于批次间变化。这项研究使用高通量的聚合物筛选方法来鉴定支持原代小鼠小脑神经元体外培养的合成聚合物。这样可以识别即使在“无血清”条件下也能使原代细胞具有高存活力的材料，