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Bioorthogonal SERS Nanotags as a Precision Theranostic Platform for in Vivo SERS Imaging and Cancer Photothermal Therapy.
Bioconjugate Chemistry ( IF 4.7 ) Pub Date : 2020-01-28 , DOI: 10.1021/acs.bioconjchem.0c00022
Jing Wang 1 , Duanwei Liang 1 , Qingqing Jin 1 , Jie Feng 1 , Xinjing Tang 1
Affiliation  

Precise detection and effective treatment are crucial to prolong cancer patients' lives. Surface-enhanced Raman scattering (SERS) imaging coupled with photothermal therapy has been considered a precise and effective strategy for cancer theranostics. Nevertheless, Raman reporters employed in the literature usually possessed multiple shift peaks in the fingerprint region, which are overlapped with background signals from endogenous biological molecules. Herein, we fabricated a new kind of bioorthogonal Raman reporter and aptamer functionalized SERS nanotags. The SERS nanotags demonstrated a strong Raman signal at 2205 cm-1 in the biologically Raman-silent region and recognized MCF-7 breast cancer cells for Raman imaging with high specificity. Laser irradiation induced serious toxicity of MCF-7 cells due to the excellent photothermal capability of the SERS nanotags. After intravenous administration of the SERS nanotags, tumor Raman spectral detection and mapping in living mice were successfully achieved. Further in vivo antitumor experiments manifested that the aptamer-modified SERS nanotags significantly restrained tumor growth after laser irradiation with 99% inhibition rate and good biocompatibility. These results clearly revealed that the SERS nanotags could serve as a novel and precise theranostic platform for in vivo cancer diagnosis and photothermal therapy.

中文翻译:

生物正交SERS纳米标签作为体内SERS成像和癌症光热疗法的精确治疗平台。

精确检测和有效治疗对于延长癌症患者的生命至关重要。表面增强拉曼散射(SERS)成像与光热疗法相结合已被认为是癌症治疗学的一种精确有效的策略。然而,文献中使用的拉曼报道分子通常在指纹区域具有多个位移峰,这些峰与来自内源性生物分子的背景信号重叠。本文中,我们制备了一种新型的生物正交拉曼报道分子和适体功能化的SERS纳米标签。SERS纳米标签在生物学拉曼沉默区域显示了在2205 cm-1处的强拉曼信号,并识别出具有高特异性的用于拉曼成像的MCF-7乳腺癌细胞。由于SERS纳米标签具有出色的光热能力,激光照射可引起MCF-7细胞严重毒性。静脉内注射SERS纳米标签后,在活体小鼠中成功实现了肿瘤拉曼光谱检测和定位。进一步的体内抗肿瘤实验表明,适体修饰的SERS纳米标签可在激光照射后以99%的抑制率和良好的生物相容性显着抑制肿瘤生长。这些结果清楚地表明,SERS纳米标签可以作为一种新颖且精确的治疗诊断平台,用于体内癌症诊断和光热疗法。进一步的体内抗肿瘤实验表明,适体修饰的SERS纳米标签可在激光照射后以99%的抑制率和良好的生物相容性显着抑制肿瘤生长。这些结果清楚地表明,SERS纳米标签可以作为一种新颖且精确的治疗诊断平台,用于体内癌症诊断和光热疗法。进一步的体内抗肿瘤实验表明,适体修饰的SERS纳米标签可在激光照射后以99%的抑制率和良好的生物相容性显着抑制肿瘤生长。这些结果清楚地表明,SERS纳米标签可以作为一种新颖且精确的治疗诊断平台,用于体内癌症诊断和光热疗法。
更新日期:2020-01-29
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