Pretreatment with white rot fungi is a promising method to enhance the digestibility of lignocelluloses; however, sterilization of feedstocks prior to inoculation is one of the costliest steps. To improve the colonizing ability of white rot fungi under non-sterile condition, Irpex lacteus, Pleurotus ostreatus, and Phanerochaete chrysosporium were inoculated in the wheat straw ensiled for 28 days and incubated for 56 days to determine the changes in microbe counts, organic acid content, chemical composition, and rumen and enzymatic digestibility. Results showed that ensiling produced abundant organic acids and suppressed most microbes in wheat straw. Significant growth of I. lacteus was observed after 3 days of incubation, and molds were only detectable at day 7 in the group. At the end of incubation, aerobic bacteria and lactic acid bacteria decreased by 18% and 38% in the wheat straw treated with I. lacteus, but molds, aerobic bacteria, and lactic acid bacteria thrived in those treated with P. ostreatus and P. chrysosporium. Even more, P. ostreatus and P. chrysosporium increased the lignin content of the ensiled wheat straw by 34% and 65%. However, I. lacteus selectively degraded lignin by 28% and improved the rumen and enzymatic digestibility by 18% and 34%. The finding indicates that ensiling prior to fermentation with I. lacteus is an effective method to control spoilage microbes and to enhance the rumen and enzymatic digestibility of wheat straw.

中文翻译：

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提高不育小麦秸秆中Irpex lacteus定殖能力的新策略，可增强瘤胃和酶消化率。

白腐真菌预处理是提高木质纤维素消化率的一种有前途的方法。但是，接种前原料的灭菌是最昂贵的步骤之一。为了提高在非无菌条件下白腐真菌的定殖能力，将青麦草，平菇和金孢菌接种到青贮的麦秸中28天，并温育56天以确定微生物计数，有机酸含量的变化。 ，化学成分以及瘤胃和酶消化率。结果表明，青贮产生了丰富的有机酸并抑制了麦秸中的大多数微生物。温育3天后观察到乳酸杆菌的显着生长，并且在该组中仅在第7天才检测到霉菌。孵化结束后，用乳酸乳球菌处理的麦草中，好氧细菌和乳酸菌分别减少了18％和38％，但用平菇和金孢假单胞菌处理的细菌中，霉菌，好氧细菌和乳酸菌蓬勃发展。更重要的是，P。ostreatus和P. chrysosporium使青贮小麦秸秆的木质素含量增加了34％和65％。但是，乳酸乳球菌选择性地将木质素降解了28％，并将瘤胃和酶消化率提高了18％和34％。该发现表明，在用乳酸乳球菌发酵之前进行铺板是控制变质微生物并增强小麦秸秆的瘤胃和酶消化率的有效方法。ostreatus和P. chrysosporium使青贮小麦秸秆的木质素含量分别增加了34％和65％。但是，乳酸乳球菌选择性地将木质素降解了28％，并将瘤胃和酶消化率提高了18％和34％。该发现表明，在用乳酸乳球菌发酵之前进行铺板是控制腐败微生物并增强小麦秸秆的瘤胃和酶消化率的有效方法。ostreatus和P. chrysosporium使青贮小麦秸秆的木质素含量分别增加了34％和65％。但是，乳酸乳球菌选择性地将木质素降解了28％，并将瘤胃和酶消化率提高了18％和34％。该发现表明，在用乳酸乳球菌发酵之前进行铺板是控制变质微生物并增强小麦秸秆的瘤胃和酶消化率的有效方法。