BACKGROUND The main objective of this study was to generate a hemodynamically stable swine model to detect spreading depolarizations (SDs) using electrocorticography (ECoG) and intrinsic optical signal (IOS) imaging and laser speckle flowmetry (LSF) after a 30-h middle cerebral artery (MCA) occlusion (MCAo) in German Landrace Swine. METHODS A total of 21 swine were used. The study comprised a training group (group 1, n = 7), a group that underwent bilateral craniectomy and MCAo (group 2, n = 10) and a group used for 2,3,5-triphenyltetrazolium (TTC) staining (group 3, n = 5). RESULTS In group 2, nine animals that underwent MCAo survived for 30 h, and one animal survived for 12 h. We detected MCA variants with 2 to 4 vessels. In all cases, all of the MCAs were occluded. The intensity changes exhibited by IOS and LSF after clipping were closely correlated and indicated a lower blood volume and reduced blood flow in the middle cerebral artery territory. Using IOS, we detected a mean of 2.37 ± (STD) 2.35 SDs/h. Using ECoG, we detected a mean of 0.29 ± (STD) 0.53 SDs/h. Infarctions were diagnosed using histological analysis. TTC staining in group 3 confirmed that the MCA territory was compromised and that the anterior and posterior cerebral arteries were preserved. CONCLUSIONS We confirm the reliability of performing live monitoring of cerebral infarctions using our MCAo protocol to detect SDs.

中文翻译：

---

猪中脑动脉闭塞模型中扩展去极化的检测。

背景技术这项研究的主要目的是建立一个血液动力学稳定的猪模型，以在大脑中动脉经过30小时后使用电皮层照相术（ECoG）和内在光信号（IOS）成像以及激光散斑血流计（LSF）来检测扩散去极化（SD）。 （MCA）堵塞（MCAo）在德国长白猪。方法共使用21头猪。该研究包括一个训练组（第1组，n = 7），一个进行了双侧颅骨切除术和MCAo的组（第2组，n = 10）和一个用于2,3,5-三苯基四唑鎓（TTC）染色的组（第3组）。 ，n = 5）。结果在第2组中，接受MCAo的9只动物存活了30小时，其中1只动物存活了12小时。我们检测到2到4艘船的MCA变体。在所有情况下，所有MCA均被遮挡。IOS和LSF剪切后显示的强度变化密切相关，表明大脑中动脉区域的血容量降低和血流减少。使用IOS，我们检测到的平均值为2.37±（STD）2.35 SDs / h。使用ECoG，我们检测到平均值为0.29±（STD）0.53 SDs / h。使用组织学分析诊断为梗塞。第3组的TTC染色证实MCA区域受到损害，并且大脑前后动脉得以保留。结论我们证实了使用MCAo协议检测SD进行脑梗死的实时监测的可靠性。使用组织学分析诊断为梗塞。第3组的TTC染色证实MCA区域受到损害，并且大脑前后动脉得以保留。结论我们证实了使用MCAo协议检测SD进行脑梗死的实时监测的可靠性。使用组织学分析诊断为梗塞。第3组的TTC染色证实MCA区域受到损害，并且大脑前后动脉得以保留。结论我们证实了使用MCAo协议检测SD进行脑梗死的实时监测的可靠性。