Traditional colorimetric sensors often suffer from the low color resolution and the not well-defined boundary between the yes and no answers, especially for distinguishing the similar color readouts with the naked eye. Herein, a simple strategy is communicated for greenly converting colorimetric sensor into a photothermal sensor, which is based on the simple color-to-thermal (temperature) signal readout conversion. The colorimetric system based on simply mixing commercially available chemicals of 3,3,5,5-tetramethylbenzidine (TMB) and silver ions (Ag⁺), provides a straightforward blue color readout owing to the generation of oxidized TMB (TMBox). In the presence of the target alkaline phosphatase (ALP), ascorbic acid phosphate (AAP) in the system could be hydrolyzed into ascorbic acid (AA), subsequently reducing the blue colored TMBox into colorless TMB. The blue-to-colorless change-based colorimetric system also exhibits the highly sensitive near-infrared (NIR) laser-driven photothermal response, allowing the successful thermometer-detectable signal-based assay for point-of-care testing (POCT) of ALP levels just using a common thermometer. This study not only demonstrated a regulatory strategy to convert color-based signal readout into thermal (temperature)-based signal readout, but also established a facile method to design affordable thermometric sensors for POCT and instrument-free bioanalysis, especially in low-resource settings.

传统的比色传感器通常会遭受色彩分辨率低和是与否答案之间边界不明确的困扰，尤其是用肉眼区分相似的颜色读数时。在此，传达了一种简单的策略，该策略基于简单的色热（温度）信号读取转换将比色传感器绿色转换为光热传感器。比色系统基于将3,3,5,5-四甲基联苯胺（TMB）和银离子（Ag ⁺），由于产生了氧化的TMB（TMBox），因此提供了直观的蓝色读数。在目标碱性磷酸酶（ALP）存在的情况下，系统中的抗坏血酸磷酸酯（AAP）可以水解为抗坏血酸（AA），随后将蓝色的TMBox还原为无色TMB。蓝色到无色的基于比色的比色系统还具有高灵敏度的近红外（NIR）激光驱动的光热响应，从而可以成功地通过温度计检测到的基于信号的分析用于ALP的即时检测（POCT）只需使用普通的温度计即可。这项研究不仅展示了一种将基于颜色的信号读数转换为基于热（温度）信号的读数的调节策略，