Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving tremendous attention for many diverse applications in the areas of industrial and medical biotechnology. Understanding the structure-function relationship of protein in IBs has recently created new possibilities in developing innovative isolation, solubilization, refolding, and purification processes for high-throughput recovery of bioactive protein from bacterial IBs. This opinion article describes the advantages, disadvantages, and major challenges presently associated with each of the processing steps. Finally, we conclude with the possible solutions for each operational step and the future direction of the basic and translational research to achieve maximum benefit from IB aggregates.

中文翻译：

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细菌包涵体：生物活性蛋白质的宝库。

表达为细菌包涵体 (IBs) 的重组蛋白现在在工业和医学生物技术领域的许多不同应用中受到极大关注。了解 IBs 中蛋白质的结构-功能关系最近为开发创新的分离、增溶、重折叠和纯化过程创造了新的可能性，以从细菌 IBs 中高通量回收生物活性蛋白。这篇意见文章描述了目前与每个处理步骤相关的优点、缺点和主要挑战。最后，我们总结了每个操作步骤的可能解决方案以及基础和转化研究的未来方向，以从 IB 聚合中获得最大收益。