BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs. The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs). METHODS Male WKAH/HkmSlc rats were fed with a control or a HFS for 13 weeks. Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively. BAs were analyzed by using ultra high-performance liquid chromatography-mass spectrometry. Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method. RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight. We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period. Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT. There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT. In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats. Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion. Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT. CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.

中文翻译：

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高脂高蔗糖饮食大鼠葡萄糖耐量试验中12α-羟基化胆汁酸与胰岛素分泌之间的关系。

背景技术以前，我们在大鼠研究中发现能量摄入与胆汁酸（BA）尤其是12α-羟基化（12αOH）BA代谢之间存在显着关系。本研究旨在通过在两种类型的葡萄糖耐量测试（GTT）中使用多元和多元回归分析揭示高脂高蔗糖饮食（HFS）喂养的大鼠BA代谢，葡萄糖耐量和盲肠有机酸之间的关系）。方法雄性WKAH / HkmSlc大鼠用对照组或HFS喂养13周。在第9周和第11周分别进行了口服葡萄糖耐量测试（OGTT）和腹膜内葡萄糖耐量测试（IPGTT）。通过使用超高效液相色谱-质谱法分析BA。使用超高效液相色谱和柱后pH缓冲电导率法分析盲肠中有机酸的浓度。结果观察到主动脉12αOHBA浓度与能量摄入和内脏脂肪组织重量呈正相关。我们发现，与对照组相比，HFS喂养的大鼠的肠肝循环，肠内容物和粪便中12αOHBAs的增加，与测试期间粪便中总BA浓度的增加无关。粪便中的12αOHBA浓度与OGTT中的最大胰岛素水平和IPGTT中的胰岛素曲线下面积呈正相关。OGTT和IPGTT中主动脉12αOHBAs浓度与血浆葡萄糖水平变化呈正相关。相反，喂食HFS的大鼠的盲肠中有机酸的浓度降低。IPGTT中的多元线性回归分析显示，主动脉12αOHBA和盲肠乙酸的浓度是胰岛素分泌的预测因子。此外，门脉12αOHBAs的浓度与IPGTT中外周血胰岛素浓度的变化呈正相关。结论伴随GTTs的BA组成的分布分析表明，12αOHBA的代谢与大鼠GTTs的胰岛素分泌密切相关。IPGTT中门脉12αOHBAs的浓度与外周血胰岛素浓度的变化呈正相关。结论伴随GTTs的BA组成的分布分析表明，12αOHBA的代谢与大鼠GTTs的胰岛素分泌密切相关。IPGTT中门脉12αOHBAs的浓度与外周血胰岛素浓度的变化呈正相关。结论伴随GTTs的BA组成的分布分析表明，12αOHBA的代谢与大鼠GTTs的胰岛素分泌密切相关。