Polyelectrolyte multilayers composed of pharmaceutical grade fucoidan and chitosan have been assembled and studied in terms of their response to physiological solution conditions and the presence of lysozyme. The influence of phosphate buffered saline (PBS) solution on the multilayer was interrogated using attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM). The combination of the techniques reveal that the polyelectrolyte multilayers swell when exposed to PBS after build-up and may include a small degree of mass loss as the film swells. The degree of swelling was influenced by the terminating layer of the multilayer. Upon exposure to lysozyme, it was observed that some deswelling occurred, as the enzyme adsorbed onto and permeated into the multilayer. The behaviour of the multilayer as a potential reservoir for lysozyme contrasts with the interaction with bovine serum albumin, which did not penetrate into the multilayer, indicating either exclusion by size or due to the overall net negative charge of the film.

中文翻译：

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在生理条件下，溶菌酶被吸收到药用级岩藻依聚糖/壳聚糖聚电解质多层膜中。

已经组装并研究了由药用级岩藻依聚糖和壳聚糖组成的聚电解质多层膜，它们对生理溶液条件的响应和溶菌酶的存在。使用衰减全反射率（ATR）傅里叶变换红外（FTIR）光谱和原子力显微镜（AFM）探究了磷酸盐缓冲盐水（PBS）溶液对多层的影响。这些技术的结合表明，聚电解质多层膜在堆积后暴露于PBS时会溶胀，并且随着膜溶胀可能会出现少量质量损失。溶胀度受多层终止层的影响。暴露于溶菌酶后，观察到发生了一些溶胀，因为酶吸附到并渗透到多层中。