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Preparation of self-assembled collagen fibrillar gel from tilapia skin and its formation in presence of acidic polysaccharides.
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2020-01-15 , DOI: 10.1016/j.carbpol.2020.115831
Mingyan Yan 1 , Xiujie Jiang 2 , Gaochao Wang 1 , Ailing Wang 1 , Xinxin Wang 1 , Xinyu Wang 1 , Xiaochen Zhao 1 , Hao Xu 1 , Xiangsheng An 1 , Yinping Li 1
Affiliation  

Fibrillar gel of pepsin-solubilized collagen from tilapia skin was prepared by self-assembly in neutral phosphate buffer at 28 °C. Then effects of acidic polysaccharides, such as sodium alginate (SA), chondroitin sulfate (CS), and hyaluronic acid (HA), on the formation and properties of self-assembled fibrillar gel were investigated. SA and CS prolonged gelling time, whereas HA had no obvious effect. SA made fibril network denser, while CS and HA induced the presence of larger ordered structures. All the acidic polysaccharides broadened the D-periodicity of fibrils. SA and HA increased the maximum mechanical strength of gel to 39.64 and 34.49 kN/m2, respectively, significantly higher than that of pure collagen gel (14.53 kN/m2), while that only 17.20 kN/m2 after CS introduced. HA had no evident effect on enzymatic resistance, while SA and CS decreased. Therefore, tilapia skin collagen with HA has a higher potential as a biomaterial than that with CS or SA.

中文翻译:

罗非鱼皮肤自组装胶原原纤维凝胶的制备及其在酸性多糖存在下的形成。

通过在28°C的中性磷酸盐缓冲液中自组装,制备罗非鱼皮肤中胃蛋白酶溶解的胶原蛋白的原纤维凝胶。然后研究了酸性多糖如藻酸钠(SA),硫酸软骨素(CS)和透明质酸(HA)对自组装原纤维凝胶的形成和性能的影响。SA和CS延长了胶凝时间,而HA没有明显的作用。SA使原纤维网络致密,而CS和HA诱导存在较大的有序结构。所有的酸性多糖都扩大了原纤维的D-周期。SA和HA将凝胶的最大机械强度分别提高到39.64 kN / m2和34.49 kN / m2,显着高于纯胶原蛋白凝胶(14.53 kN / m2),而在CS引入后仅达到17.20 kN / m2。HA对酶抗性没有明显影响,而SA和CS下降。因此,具有HA的罗非鱼皮肤胶原作为生物材料比具有CS或SA的罗非鱼皮肤胶原具有更高的潜力。
更新日期:2020-01-15
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