In this research, cellulose nanocrystal (CNC) was synthesized from cotton waste using controlled hydrolysis against 64 % (w/w) sulfuric acid solution. The produced nanoparticles were then characterized using FTIR, XRD, TGA, and DLS analyses. Biaxial electrospinning technique was used to produce CNC incorporated PCL-PVA/NaAlg nanofibers. The sodium alginate portion was then crosslinked via submerging the samples in calcium chloride aqueous solution. The CNC incorporated and crosslinked sample was characterized using SEM, FTIR, and TGA techniques. Results confirmed the presence of CNC nanoparticles and alginate crosslinking reaction. Mechanical studies showed that CNC incorporation increases the tensile modulus by 65 %. Also, the crosslinked samples exhibited an increase in elongation at break. Water contact angle studies suggested that CNC incorporation and crosslinking improves nanofiber hydrophilicity. Cell viability of more than 90 % was observed in CNC incorporated PCL-CaAlg nanofibers. Also, SEM images of cells on nanofiber scaffolds showed better cell growth and attachment in PCL-CaAlg-CNC samples.

中文翻译：

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通过掺入纤维素纳米晶体来制造具有增强的生物力学性能的藻酸盐/聚己内酯纳米纤维。

在这项研究中，使用针对64％（w / w）的硫酸溶液进行受控水解，由棉花废料合成了纤维素纳米晶体（CNC）。然后使用FTIR，XRD，TGA和DLS分析对产生的纳米颗粒进行表征。双轴静电纺丝技术用于生产结合了CNC的PCL-PVA / NaAlg纳米纤维。然后通过将样品浸没在氯化钙水溶液中使海藻酸钠部分交联。使用SEM，FTIR和TGA技术对CNC结合和交联的样品进行表征。结果证实了CNC纳米颗粒和藻酸盐交联反应的存在。力学研究表明，加入CNC可以将拉伸模量提高65％。而且，交联的样品表现出断裂伸长率的增加。水接触角研究表明，CNC的引入和交联可提高纳米纤维的亲水性。在并入CNC的PCL-CaAlg纳米纤维中观察到细胞活力超过90％。同样，纳米纤维支架上细胞的SEM图像显示PCL-CaAlg-CNC样品中细胞生长和附着性更好。