SNP loci linked to the crown rust resistance gene Pc98 were identified by linkage analysis and KASP assays were developed for marker-assisted selection in breeding programs. Crown rust is among the most damaging diseases of oat and is caused by Puccinia coronata var. avenae f. sp. avenae (Urban and Marková) (Pca). Host resistance is the preferred method to prevent crown rust epidemics. Pc98 is a race-specific, seedling crown rust resistance gene obtained from the wild oat Avena sterilis accession CAV 1979 that is effective at all growth stages of oat. Virulence to Pc98 has been very low in the Pca populations that have been tested. The objectives of this study were to develop SNP markers linked to Pc98 for use in marker-assisted selection and to locate Pc98 on the oat consensus map. The Pc98 gene was mapped using F2:3 populations developed from the crosses Pc98/Bingo and Pc98/Kasztan, where Pc98 is a single-gene line carrying Pc98. Both populations were evaluated in seedling inoculation experiments. Pc98 was mapped relative to Kompetitive Allele-Specific PCR SNP markers in both populations, placing Pc98 on the Mrg20 linkage group of the consensus map. Pc98 was bracketed by two SNP markers GMI_ES22_c3052_382_kom399 and GMI_ES14_lrc18344_662_kom398 in the Pc98/Bingo mapping population with genetic distances of 0.9 cM and 0.3 cM, respectively. Pc98 co-segregated with four SNP markers in the Pc98/Kasztan population, and the closest flanking markers were GMI_DS_LB_6017_kom367 and avgbs2_153634.1.59_kom410 with genetic distances of 0.7 cM and 0.3 cM, respectively. Two SNP loci defined a haplotype that accurately predicted Pc98 status in a diverse group of oat germplasm, which will be valuable for marker-assisted selection of Pc98 in breeding of new oat cultivars.

中文翻译：

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栽培燕麦（Avena sativa L.）抗冠锈病基因Pc98的染色体定位。

通过连锁分析鉴定了与抗冠锈病基因 Pc98 连锁的 SNP 基因座，并开发了 KASP 测定法用于育种计划中的标记辅助选择。冠锈病是燕麦最具破坏性的病害之一，由 Puccinia coronata var 引起。avenae f. sp。avenae (Urban and Marková) (Pca)。宿主抗性是预防冠锈病流行的首选方法。Pc98 是一种从野生燕麦 Avena sterilis accession CAV 1979 中获得的种种特异性幼苗抗锈病基因，在燕麦的所有生长阶段均有效。在已测试的 Pca 种群中，对 Pc98 的毒力非常低。本研究的目的是开发与 Pc98 相关的 SNP 标记，用于标记辅助选择，并在燕麦共有图谱上定位 Pc98。使用 F2 绘制 Pc98 基因图谱：Pc98/Bingo和Pc98/Kasztan杂交产生3个种群，其中Pc98是携带Pc98的单基因系。两个种群都在幼苗接种实验中进行了评估。Pc98 相对于两个群体中的竞争性等位基因特异性 PCR SNP 标记进行定位，将 Pc98 置于共有图谱的 Mrg20 连锁群上。Pc98 在遗传距离分别为 0.9 cM 和 0.3 cM 的 Pc98/Bingo 作图群体中被两个 SNP 标记 GMI_ES22_c3052_382_kom399 和 GMI_ES14_lrc18344_662_kom398 括起来。Pc98 与 Pc98/Kasztan 群体中的四个 SNP 标记共分离，最接近的侧翼标记是 GMI_DS_LB_6017_kom367 和 avgbs2_153634.1.59_kom410，遗传距离分别为 0.7 cM 和 0.3 cM。