This study evaluates the influence of prebiotic carbohydrates, namely fructooligosaccharides (FOS) and galactooligosaccharides (GOS), on the protein expression of Enterococcus durans LAB18S. The strain was cultivated in 10 g L^-1 FOS, GOS or glucose (control) and cellular proteins were extracted for mass spectrometry analysis. A total of 771 proteins were identified and 135 E. durans proteins were validated by the Scaffold algorithm. The proteins were functionally categorized according to Gene Ontology terms. Both FOS and GOS were used as carbon source by E. durans LAB18S, upregulating the production of proteins that may be associated with intestinal mucosa adhesion, carbohydrate and nitrogen metabolism, and stress response. Cells grown with GOS showed an increased expression of the cell division protein divIVA, EF-Tu and glyceraldehyde 3-phosphate dehydrogenase that have been associated with epithelial cell adhesion. The use of FOS stimulated the production of proteins related to amino acid metabolism and energy conversion, and ClpX protein, which plays an important role in protein turnover. The results of this study indicate that FOS and GOS can be metabolized by E. durans and stimulate the microorganism to produce proteins related to some desirable characteristics for a probiotic strain.

这项研究评估了益生元碳水化合物，即低聚果糖（FOS）和低聚半乳糖（GOS），对杜兰肠球菌LAB18S蛋白质表达的影响。该菌株在10 g L ^-1 FOS，GOS或葡萄糖（对照）中培养，并提取细胞蛋白进行质谱分析。通过Scaffold算法，共鉴定了771种蛋白质，并验证了135种杜兰大肠杆菌。根据基因本体论术语对蛋白质进行功能分类。FOS和GOS均被杜兰E. durans用作碳源LAB18S，上调可能与肠粘膜黏附，碳水化合物和氮代谢以及应激反应有关的蛋白质的产生。用GOS生长的细胞显示出与上皮细胞粘附相关的细胞分裂蛋白divIVA，EF-Tu和3-磷酸甘油醛脱氢酶的表达增加。FOS的使用刺激了与氨基酸代谢和能量转换有关的蛋白质的产生，而ClpX蛋白质在蛋白质更新中起着重要作用。这项研究的结果表明FOS和GOS可以被杜兰肠杆菌（E. durans）代谢并刺激微生物产生与益生菌菌株某些理想特性相关的蛋白质。