Abstract Herein, we report on a sensitive probe for colorimetric detection of uric acid based on the formation of Ag shell on Au nanorods (Ag/AuNRs) in the presence of uric acid. Uric acid can reduce Ag+ to Ag atoms which deposit on the surface of AuNRs and form Ag shell. As a result of this phenomenon, the dielectric environment around AuNRs changes and the longitudinal surface plasmon resonance (SPR) peak of AuNRs blue-shifts, leading to the change of solution color from purple to green. Based on these facts, a colorimetric sensor for the detection of uric acid in the concentration range of 0.1–1.0 µM with a detection limit of 0.065 µM was developed. The method was applied for analysis of uric acid in human plasma and urine samples with satisfactory results. The sensor can be used for uric acid detection even with bare eyes.

中文翻译：

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原位形成 Ag/Au 纳米棒作为设计用于生物体液中尿酸检测的非聚集比色测定的平台

摘要在此，我们报告了一种基于在尿酸存在下在 Au 纳米棒 (Ag/AuNRs) 上形成 Ag 壳的用于比色检测尿酸的灵敏探针。尿酸可以将 Ag+ 还原为 Ag 原子，Ag 原子沉积在 AuNRs 表面并形成 Ag 壳。由于这种现象，AuNRs 周围的介电环境发生变化，AuNRs 的纵向表面等离子体共振 (SPR) 峰蓝移，导致溶液颜色从紫色变为绿色。基于这些事实，开发了一种用于检测浓度范围为 0.1–1.0 µM、检测限为 0.065 µM 的尿酸的比色传感器。将该方法应用于人血浆和尿样中尿酸的分析，结果满意。即使裸眼，该传感器也可用于尿酸检测。