Abstract Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation.

中文翻译：

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从具有降解商业 B10 柴油能力的沼气厂获得的兼性残留污泥的微生物特征

摘要 首先研究了存在于沼气残留污泥中的微生物群落对商业 B10 柴油 (DB10) 的生物降解。DB10 的石化和脂肪酸甲酯 (FAME) 馏分的最大去除效率分别为 55 ± 3% 和 94 ± 6%。对残留污泥中存在的微生物群落进行分类分析，以确定潜在的碳氢化合物降解细菌。在使用 DB10 进行温和的选择压力后，在处理过的样品中主要发现了三种假单胞菌物种（铜绿假单胞菌、斯氏假单胞菌和门多克假单胞菌）。然而，只有铜绿假单胞菌和施氏假单胞菌以 DB10 作为唯一碳源为代价显示出快速生长和高脱氢酶活性。此外，两种菌株都去除了 42 ± 5% 和 53 ± 2% 的石化成分，并且 > 分别为 FAME 分数的 75%。在三个分离的假单胞菌的细菌聚生体中发现了类似的结果，表明这些菌株没有协同降解碳氢化合物。正如预期的那样，假单胞菌产生的生物表面活性剂与细菌 DB10 的生物降解性能直接相关。这些结果首次描述了从沼气设施中获得的粗兼性残余污泥的石油基烃生物降解能力；并代表了理性的第一步，以了解污泥中的哪些细菌可能会在石油基燃料降解中起作用。假单胞菌产生的生物表面活性剂与细菌 DB10 的生物降解性能直接相关。这些结果首次描述了从沼气设施中获得的粗兼性残余污泥的石油基烃生物降解能力；并代表了理性的第一步，以了解污泥中的哪些细菌可能会在石油基燃料降解中起作用。假单胞菌产生的生物表面活性剂与细菌 DB10 的生物降解性能直接相关。这些结果首次描述了从沼气设施中获得的粗兼性残余污泥的石油基烃生物降解能力；并代表了理性的第一步，以了解污泥中的哪些细菌可能会在石油基燃料降解中起作用。