This work aims to evaluate the effects of temperature and pressure on the extraction yield and chemical composition of the supercritical CO₂ (SCCO₂) extracts of A. gratissima leaves (EAG) and then to investigate in vivo anti-inflammatory activity of the extracts. The leaves of A. gratissima were extracted with supercritical CO₂ at 40, 50 and 60 °C and 15, 17.5 and 20 MPa for 120 min. The anti-inflammatory activity was performed with the EAG obtained at 60 °C and 20 MPa. The anti-inflammatory activity was investigated by the carrageenan-induced paw edema and myeloperoxidase (MPO) activity in paw tissue. The chemical analysis showed the presence of guaiol, pinocamphone, caryophyllene oxide and spathulenol as the major chemical components of the EAG. These compounds may have contributed to the significant reduction of carrageenan-induced paw edema and MPO activity in the first 3 h after the injury induction.

这项工作旨在评估温度和压力对超临界CO的提取率和化学组成的影响₂（SCCO ₂）提取物的A. gratissima叶（EAG），然后，调查在体内提取物的抗炎活性。用超临界CO ₂提取紫草的叶片在40、50和60°C以及15、17.5和20 MPa的压力下持续120分钟。用在60°C和20 MPa下获得的EAG进行抗炎活性。通过角叉菜胶诱发的爪水肿和爪组织中的髓过氧化物酶（MPO）活性研究了抗炎活性。化学分析表明，番石榴酚，松果油烟醚，石竹烯氧化物和芦荟酚是EAG的主要化学成分。这些化合物可能在诱导损伤后的最初3小时内显着降低了角叉菜胶诱导的爪水肿和MPO活性。