In the last years, antimicrobial photodynamic therapy (aPDT) has been considered as one important alternative to be used in antimicrobial treatments. In the present work we report the first photoinactivation of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) by cationic free-base meso-tetra-(phenyl)porphyrin and its Zn(II) complex containing peripheral Ru(II)-bipyridyl complexes. Bacterial inoculum (10⁷ CFU mL^-1) was incubated with the porphyrins (25 μM) with an irradiation system containing a halogen lamp with fluence values of 0, 180, 360, 720 and 1080 J cm^-2. Both porphyrins demonstrate phototoxicity against bacteria, but higher efficiency was observed by Zn(II) complex. It was demonstrated that RuTPyP and ZnRuTPyP phototoxicity is due to their photodynamic activity associated with the formation of singlet oxygen, anion superoxide and hydroxyl radical at their irradiation by visible light. The formation of these reactive species was confirmed using flash-photolysis technique and spin-trap electron paramagnetic resonance (EPR) spectroscopy. The singlet oxygen quantum yield was determined by indirect method using the uric acid as singlet oxygen scavenger. Besides the characterization of optical absorption and fluorescence RuTPyP and ZnRuTPyP properties were realized using optical absorption and fluorescence spectroscopies. It was observed that complexation with Zn(II) ion increases the quantum yields of porphyrin fluorescence and singlet oxygen formation.

近年来，抗菌光动力疗法（aPDT）被认为是用于抗菌治疗的一种重要替代方法。在本工作中，我们报道了阳离子游离碱内消旋-四-（苯基）卟啉及其含有外围Ru（II）-联吡啶基配合物的Zn（II）配合物对肠炎沙门氏菌鼠伤寒沙门氏菌（Salmonella Typhimurium）的首次光灭活。将细菌接种物（10 ⁷ CFU mL ^-1）与卟啉（25μM）在装有卤素灯的辐照系统中孵育，通量值为0、180、360、720和1080 J cm ^-2。两种卟啉均显示出对细菌的光毒性，但通过Zn（II）络合物观察到更高的效率。证明RuTPyP和ZnRuTPyP的光毒性是由于它们的光动力活性与可见光照射下单线态氧，阴离子超氧化物和羟基自由基的形成有关。这些反应物种的形成已使用闪光灯光解技术和自旋陷阱电子顺磁共振（EPR）光谱进行了确认。通过使用尿酸作为单线态氧清除剂的间接方法确定单线态氧量子产率。除了表征光吸收和荧光RuTPyP和ZnRuTPyP使用光吸收和荧光光谱学来实现性质。观察到与Zn（II）离子的络合增加了卟啉荧光和单线态氧形成的量子产率。