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Human mesenchymal stem cells encapsulated-coacervated photoluminescent nanodots layered bioactive chitosan/collagen hydrogel matrices to indorse cardiac healing after acute myocardial infarction
Journal of Photochemistry and Photobiology B: Biology ( IF 5.4 ) Pub Date : 2020-01-15 , DOI: 10.1016/j.jphotobiol.2020.111789
Rui Si , Chao Gao , Rui Guo , Chen Lin , Jiayi Li , Wenyi Guo

Acute Myocardial Infarction (MI) is one of the foremost causes of human death worldwide and it leads to mass death of cardiomyocytes, interchanges of unfavorable biological environment and affecting electrical communications by fibrosis scar formations, and specifically deficiency of blood supply to heart which leads to heart damage and heart failure. Recently, numerous appropriate strategies have been applied to base on solve these problems wound be provide prominent therapeutic potential to cardiac regeneration after acute MI. In the present study, a combined biopolymeric conductive hydrogel was fabricated with conductive ultra-small graphene quantum dots as a soft injectable hydrogel for cardiac regenerations. The resultant hydrogel was combined with human Mesenchymal stem cells (hMSCs) to improved angiogenesis in cardiovascular tissues and decreasing cardiomyocyte necrosis of hydrogel treated acute-infarcted region has been greatly associated with the development of cardiac functions in MI models. The prepared graphene quantum dots and hydrogel groups was physico-chemically analyzed and confirmed the suitability of the materials for cardiac regeneration applications. The in vitro analyzes of hydrogels with hMSCs have established that enhanced cell survival rate, increased expressions of pro-inflammatory factors, pro-angiogenic factors and early cardiogenic markers. The results of in vivo myocardial observations and electrocardiography data demonstrated a favorable outcome of ejection fraction, fibrosis area, vessel density with reduced infarction size, implying that significant development of heart regenerative function after MI. This novel strategy of injectable hydrogel with hMSCs could be appropriate for the effective treatment of cardiac therapies after acute MI.



中文翻译:

人间充质干细胞包裹的凝聚光致发光纳米点分层的生物活性壳聚糖/胶原水凝胶基质可在急性心肌梗死后促进心脏愈合

急性心肌梗塞(MI)是全世界人类死亡的最主要原因之一,它导致心肌细胞大量死亡,不利的生物环境互换并通过纤维化疤痕形成影响电通讯,尤其是心脏血液供应不足,从而导致心脏损害和心力衰竭。近来,基于解决这些问题,已经应用了许多适当的策略来为急性心肌梗死后的心脏再生提供突出的治疗潜力。在本研究中,结合了导电性超小石墨烯量子点的生物聚合导电水凝胶作为用于心脏再生的软质可注射水凝胶。将所得的水凝胶与人间充质干细胞(hMSCs)结合以改善心血管组织中的血管生成,并且水凝胶治疗的急性梗塞区的心肌细胞坏死的减少与MI模型中心脏功能的发展密切相关。对所制备的石墨烯量子点和水凝胶基团进行了物理化学分析,证实了该材料适用于心脏再生。的用hMSC对水凝胶进行的体外分析已确定,可以提高细胞存活率,增加促炎因子,促血管生成因子和早期心源性标志物的表达。结果体内心肌观察和心电图数据显示射血分数,纤维化面积,降低心肌梗死面积血管密度的一个有利的结果,这意味着心肌梗死后心脏再生功能的显著发展。hMSCs注射水凝胶的这种新策略可能适用于急性MI后心脏疗法的有效治疗。

更新日期:2020-01-15
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