Microalgal cell wall integrity and composition have a significant impact on the fermentation process and biofuel recovery. In this study, various biofuels (bioethanol, higher alcohols (C3-C5), and biodiesel) were produced by the fermentation of carbohydrates and proteins, and transesterification of lipids from three different microalgal strains (Pseudochlorella sp., Chlamydomonas mexicana, and Chlamydomonas pitschmannii), each possessing different proportions of bioconstituents (carbohydrates, proteins, and lipids). Changes in the cell wall structure and thickness were observed before and after fermentation using transmission electron microscopy. Pseudochlorella sp. showed the highest yields of bioethanol (0.45 g-ethanol/g-carbohydrates), higher alcohols (0.44 g-higher alcohols/g-proteins), and biodiesel (0.55 g-biodiesel/g-lipids), which consequently revealed a maximum energy recovery (42%) from whole constituents. This study suggests that different physiological properties, including cell wall thickness and the proportion of bioconstituents in microalgae, could have a significant impact on the pretreatment and fermentation efficiencies for biofuels production.

中文翻译：

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在预处理和发酵过程中，基于生物成分的微藻转化为生物燃料。

微藻细胞壁的完整性和组成对发酵过程和生物燃料回收有重大影响。在这项研究中，通过碳水化合物和蛋白质的发酵，以及来自三种不同微藻菌株（假单胞菌属，衣原体衣藻和衣原体衣藻）的脂质的酯交换作用，产生了各种生物燃料（生物乙醇，高级醇（C3-C5）和生物柴油）。 ），每种都具有不同比例的生物成分（碳水化合物，蛋白质和脂质）。使用透射电子显微镜观察发酵前后细胞壁结构和厚度的变化。假小球藻 显示出最高的生物乙醇产量（0.45 g-乙醇/ g碳水化合物），高级醇（0.44 g-高级醇/ g蛋白质）和生物柴油（0.55 g-生物柴油/ g脂质），结果表明，从全部成分中回收的能量最大（42％）。这项研究表明，不同的生理特性，包括细胞壁厚度和微藻中生物成分的比例，可能对生物燃料生产的预处理和发酵效率产生重大影响。