Periosteum as an important component in the construct of bone is mainly responsible for providing nourishment and regulating osteogenic differentiation. When bone defect happens, the functionality of periosteum will also be influenced, furthermore, it will finally hamper the process of bone regeneration. However, fabrication of an artificial periosteum with the capabilities in accelerating angiogenesis and osteogenesis in the defect area is still a challenge for researchers. In this study, we fabricated an organic-inorganic hybrid biomimetic periosteum by electrospinning, which can induce mineralization in situ and control the ions release for long-term in local area. Further, this system exhibited potential capabilities in promoting in vitro, which means the potentiality in accelerating bone regeneration in vivo. Calcium phosphate nanoparticles (CaPs) were fabricated by emulsion method, then CaPs were further incorporated with gelatin-methacryloyl (GelMA) by electrospinning fibers to construct the hybrid hydrogel fibers. The fibers exhibited satisfactory morphology and mechanical properties, additionally, controlled ions release could be observed for over 10 days. Further, significant mineralization was proved on the surface of hybrid fibers after 7 days and 14 days' co-incubation with simulated body fluid (SBF). Then, favorable biocompatibility of the hybrid fibers was approved by co-cultured with MC3T3-E1 cells. Finally, the hybrid fibers exhibited potential capabilities in promoting angiogenesis and osteogenesis by co-culture with HUVECs and MC3T3-E1 cells. This biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum provided a promising strategy to develop periosteum biomaterials with angiogenesis and osteogenesis capabilities.

骨膜作为骨骼结构中的重要组成部分，主要负责提供营养并调节成骨分化。当发生骨缺损时，骨膜的功能也将受到影响，此外，它将最终阻碍骨再生的过程。然而，制造具有能够促进缺损区域的血管生成和成骨的能力的人工骨膜仍然是研究人员面临的挑战。在这项研究中，我们通过静电纺丝制备了一种有机-无机混合仿生骨膜，可以诱导原位矿化并控制离子在局部的长期释放。此外，该系统在体外促进中表现出潜在的能力，这意味着在体内加速骨再生的潜力。通过乳液法制备了磷酸钙纳米粒子（CaPs），然后通过电纺纤维将CaPs与明胶-甲基丙烯酰基（GelMA）进一步掺混，以构建杂化水凝胶纤维。纤维表现出令人满意的形态和机械性能，此外，可以观察到超过10天的受控离子释放。此外，在与模拟体液（SBF）共孵育7天和14天后，杂化纤维的表面被证明具有明显的矿化作用。然后，通过与MC3T3-E1细胞共培养，证实了杂化纤维的良好生物相容性。最后，杂化纤维通过与HUVEC和MC3T3-E1细胞共培养，具有促进血管生成和成骨的潜在能力。