Abstract After an osteosarcoma excision, recurrence, large bone defects, and soft tissue injury are significant challenges for clinicians. Conventional treatment by implanting bone replacement materials can induce bone regeneration after surgery, but this does not prevent bleeding, promote soft tissue repair, or help destroy the residual tumor cells. We attempted to develop a new multifunctional scaffold, with the clinical goals of facilitating tumor cell death through thermal ablation and promoting osteogenesis. Accordingly, we first investigated the effect of nano-hydroxyapatite/graphene oxide (nHA/GO) composite particles with different proportions on human osteosarcoma cells (HOS), pre-osteoblastic MC3T3-E1 cells, and human bone marrow mesenchymal stem cells (hBMSC) with or without 808-nm near-infrared (NIR) light irradiation. Next, we fabricated a novel temperature-controlled multifunctional nano-hydroxyapatite/graphene oxide/chitosan (nHA/GO/CS) scaffold, which can effectively kill human osteosarcoma cells under 808-nm NIR irradiation by reaching a temperature of 48 °C and further promote osteogenesis of hBMSC at 42 ± 0.5 °C in coordination with nHA. This scaffold demonstrates the best post-operative bone volume/tissue volume (BV/TV) ratio performance (20.36%) 8 weeks after scaffold implantation in the cranial defects of rats. Further exploration has revealed that NIR irradiation may promote the osteogenesis of hBMSC with the addition of nHA by enhancing the BMP2/Smad signaling pathway. Further, this scaffold has a good hemostatic effect and facilitates soft tissue repair under irradiation. This novel photothermally controlled multifunctional scaffold, which not only kills human osteosarcoma cells but also facilitates tissue regeneration, is a promising clinical tool for treating tissue injuries from an osteosarcoma resection.

中文翻译：

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一种用于骨肉瘤临床治疗和组织再生的新型光热控制多功能支架

摘要 骨肉瘤切除术后，复发、大骨缺损和软组织损伤是临床医生面临的重大挑战。通过植入骨替代材料的常规治疗可以在手术后诱导骨再生，但这并不能防止出血、促进软组织修复或帮助破坏残留的肿瘤细胞。我们试图开发一种新的多功能支架，其临床目标是通过热消融促进肿瘤细胞死亡和促进成骨。因此，我们首先研究了不同比例的纳米羟基磷灰石/氧化石墨烯（nHA/GO）复合颗粒对人骨肉瘤细胞（HOS）、前成骨细胞MC3T3-E1细胞和人骨髓间充质干细胞（hBMSC）的影响有或没有 808 纳米近红外 (NIR) 光照射。下一个，我们制造了一种新型温控多功能纳米羟基磷灰石/氧化石墨烯/壳聚糖（nHA/GO/CS）支架，在808-nm NIR照射下通过达到48°C的温度有效杀死人骨肉瘤细胞并进一步促进成骨hBMSC 在 42 ± 0.5 °C 与 nHA 协调。该支架在支架植入大鼠颅骨缺损后 8 周表现出最佳的术后骨体积/组织体积 (BV/TV) 比性能 (20.36%)。进一步的探索表明，NIR 照射可能通过增强 BMP2/Smad 信号通路，在添加 nHA 的情况下促进 hBMSC 的成骨。此外，该支架具有良好的止血效果，有利于照射下的软组织修复。这种新型光热控制多功能支架，