Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, often pose a serious risk not only when delivered in the bloodstream but also in air, the environment and several industrial fields such as pharmaceutics or food. LPS is constituted of three regions; the O-specific chain, the core region and the lipid A, which is the responsible segment of the toxicity. Previous literature dealt with the study of lipid A, its potential ligands as well as the mechanisms of Lipid A interactions that, among other applications, establish the basis for detection methods such as Limulus Amebocyte Lysate (LAL) assays and emerging biosensoring techniques. However, quantifying LPS binding affinity is an urgent need that still requires thorough studies. In this context, this work reviews the molecules that bind LPS, highlighting quantitative affinity parameters. Moreover, state of the art methods to analyze the affinity and kinetics of lipid-ligand interactions are also reviewed and different techniques have been briefly described. Thus, first, we review existing information on LPS ligands, classifying them into three main groups and targeting the comparison of molecules in terms of their interaction affinities and, second, we establish the basis for further research aimed at the development of effective methods for LPS detection and removal.

中文翻译：

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LPS和LPS结合分子之间的生化相互作用。

脂多糖（LPS）是革兰氏阴性细菌外膜的主要成分，不仅在血液中，在空气，环境和制药或食品等多个工业领域中传递时，经常构成严重风险。LPS由三个区域组成；O特异性链，核心区域和脂质A是毒性的主要部分。先前的文献涉及脂质A及其潜在配体的研究以及脂质A相互作用的机制，这些研究在其他应用中为establish方法（如Li变形细胞溶解液（LAL）测定法和新兴的生物传感技术）奠定了基础。但是，量化LPS结合亲和力是迫切需要，仍然需要进行深入研究。在这种情况下，这项工作回顾了结合LPS的分子，突出定量亲和力参数。此外，还回顾了分析脂质-配体相互作用的亲和力和动力学的最新技术，并简要描述了不同的技术。因此，首先，我们审查有关LPS配体的现有信息，将其分为三个主要组，并针对分子之间的亲和力进行分子比较，其次，我们为进一步研究奠定基础，以开发有效的LPS方法检测和删除。