Platelet‐rich fibrin (PRF) was prepared from the blood of BALB/C inbred mice to explore potential effects on postoperative intestinal adhesion. A murine model of intestinal adhesion characterized by abdominal wall defect/and cecum damage was established by scraping caecum serosa and cutting peritoneum and muscles in the abdominal wall. The wound was covered with PRF (group A), sodium hyaluronate (group B), or left alone (blank control; group C). All animals were monitored for 28 days. The incidence of adhesion was 35.0, 66.7, and 73.7% in groups A, B, and C, respectively. The incidence of adhesion in group A was significantly lower than that in group C (p < .05). Histopathologically, severity of fibrosis and the number of fibroblasts or inflammatory cells in group A were lower than those in groups B and C (p < .05), whereas the number of mesothelial cells was higher (p = .001). Furthermore, the severity of fibrosis and number of fibroblasts or inflammatory cells were lower in low grade than those in high grade of adhesion (p < .05), whereas the number of mesothelial cells was higher (p < .05). Collectively, PRF applied to abdominal surgery may reduce the incidence of intestinal adhesion by promoting proliferation of mesothelial cells whereas inhibiting proliferation of fibroblasts and infiltration of inflammatory cells.

中文翻译：

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富含血小板的纤维蛋白可防止术后肠粘连。

从 BALB/C 近交系小鼠的血液中制备富含血小板的纤维蛋白 (PRF)，以探索对术后肠粘连的潜在影响。以腹壁缺损/盲肠损伤为特征的小鼠肠粘连模型，通过刮盲盲肠浆膜，切开腹膜和腹壁肌肉，建立小鼠肠粘连模型。伤口用PRF（A组）、透明质酸钠（B组）覆盖，或单独留下（空白对照；C组）。监测所有动物28天。A、B、C组粘连发生率分别为35.0%、66.7%和73.7%。A组粘连发生率显着低于C组（p  < .05）。组织病理学上，A组的纤维化严重程度和成纤维细胞或炎症细胞的数量低于B组和C组。p  < .05），而间皮细胞的数量更高（p = .001）。此外，低级别的纤维化严重程度和成纤维细胞或炎症细胞的数量低于高级别粘连（p  < .05），而间皮细胞数量更高（p  < .05）。总的来说，应用于腹部手术的PRF可以通过促进间皮细胞增殖、抑制成纤维细胞增殖和炎症细胞浸润来降低肠粘连的发生率。