Multicolour structured illumination microscopy (SIM) is a powerful tool used for the investigation of the dynamic interaction between subcellular structures. Nevertheless, most of the multicolour SIM schemes are currently limited by conventional fluorescent dyes and wavelength‐dependent optical systems, and can only sequentially record images of different colour channels instead of obtaining multicolour datasets simultaneously. To address these issues, we present a novel multicolour SIM scheme referred to as quantum dot structured illumination microscopy (QD‐SIM). QD‐SIM enables simultaneously excitation and collection of multicolour fluorescent signals. We also propose a theoretical analysis of the image formation in two‐dimensional multicolour SIM to help combine the optically sectioned and super‐resolution attributes of SIM. Based on this theory, QD‐SIM enables optically sectioned, super‐resolution, multicolour simultaneous imaging at a single plane.

中文翻译：

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使用量子点结构照明显微镜的同时多色成像

多色结构照明显微镜 (SIM) 是一种强大的工具，用于研究亚细胞结构之间的动态相互作用。尽管如此，目前大多数多色 SIM 方案都受到传统荧光染料和波长相关光学系统的限制，只能顺序记录不同颜色通道的图像，而不能同时获得多色数据集。为了解决这些问题，我们提出了一种新的多色 SIM 方案，称为量子点结构照明显微镜 (QD-SIM)。QD-SIM 能够同时激发和收集多色荧光信号。我们还提出了二维多色 SIM 图像形成的理论分析，以帮助结合 SIM 的光学切片和超分辨率属性。