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Oligotrophic bacterium Hymenobacter latericoloratus CGMCC 16346 degrades the neonicotinoid imidacloprid in surface water.
AMB Express ( IF 3.7 ) Pub Date : 2020-01-14 , DOI: 10.1186/s13568-019-0942-y
Leilei Guo 1 , Zhiling Dai 1 , Jingjing Guo 1 , Wenlong Yang 1 , Feng Ge 2 , Yijun Dai 1
Affiliation  

The intensive and extensive application of imidacloprid in agriculture has resulted in water pollution and risks to aquatic invertebrates. However, pure bacteria remediation of imidacloprid in surface water environments has not been studied. Here, we isolated an imidacloprid-degrading bacterium from a water environment, examined its imidacloprid degradation in pure culture and surface water, sequenced its genome, and compared its Clusters of Orthologous Groups (COG) protein categorization with that for another imidacloprid-degrading bacterium. The isolate was an obligate oligotrophic bacterium, Hymenobacter latericoloratus CGMCC 16346, which degraded imidacloprid via hydroxylation by co-metabolism in pure culture. Resting cells degraded 64.4% of 100 mg/L imidacloprid in 6 days in the presence of co-substrate maltose, and growing culture degraded 40.8% of imidacloprid in 10 days. H. latericoloratus CGMCC 16346 degraded imidacloprid in surface water without co-substrate supplementation and retained imidacloprid-degrading activity after 30 days. The half-life of imidacloprid in surface water was decreased from 173.3 days in the control to 57.8 days by CGMCC 16346 inoculation. Genome sequencing and COG analysis indicated that carbohydrate metabolism and transport, cell wall/membrane biogenesis, and defense mechanisms are enriched in H. latericoloratus CGMCC 16346 compared with the copiotrophic imidacloprid-degrading Pseudoxanthomonas indica CGMCC 6648, indicating that H. latericoloratus CGMCC 16346 is adapted to live in oligotrophic water environments and biofilms. H. latericoloratus CGMCC 16346 is a promising bioremediation agent for elimination of imidacloprid contamination from surface water.

中文翻译:

寡养细菌latemecoloratus CGMCC 16346降解了地表水中的新烟碱类吡虫啉。

吡虫啉在农业中的广泛广泛应用已导致水污染和水生无脊椎动物的风险。然而,尚未研究地表水环境中吡虫啉的纯细菌修复。在这里,我们从水环境中分离出了一种吡虫啉降解细菌,在纯培养液和地表水中检查了其吡虫啉降解情况,对其基因组进行了测序,并将其直系同源簇(COG)蛋白质分类与另一种吡虫啉降解细菌进行了比较。该分离物是专性的贫营养细菌,Hymenobacter Latericoloratus CGMCC 16346,其通过纯培养中的共代谢通过羟基化降解吡虫啉。在存在共底物麦芽糖的情况下,静息细胞在6天内降解了100 mg / L吡虫啉的64.4%,生长的培养物在10天内降解了吡虫啉40.8%。嗜血双歧杆菌CGMCC 16346在没有共同底物补充的情况下在地表水中降解了吡虫啉,并在30天后保留了吡虫啉降解活性。通过CGMCC 16346接种,吡虫啉在地表水中的半衰期从对照组的173.3天减少到57.8天。基因组测序和COG分析表明,与降解嗜热吡虫啉的伪拟黄单胞菌CGMCC 6648相比,latercolorcolorus CGMCC 16346丰富了碳水化合物的代谢和运输,细胞壁/膜生物发生和防御机制,表明适应了Latericoloratus CGMCC 16346生活在贫营养的水环境和生物膜中。H。
更新日期:2020-01-14
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