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α-Lactalbumin and chitosan core-shell nanoparticles: resveratrol loading, protection, and antioxidant activity.
Food & Function ( IF 6.1 ) Pub Date : 2020-01-29 , DOI: 10.1039/c9fo01998g
Yuexiang Liu 1 , Luyu Gao , Jiang Yi , Yuting Fan , Xuli Wu , Yuzhu Zhang
Affiliation  

Resveratrol (RES)-loaded protein-polysaccharide nanoparticles were fabricated through simple electrostatic interactions with oppositely charged α-lactalbumin (ALA) and chitosan (CHI) with a mass ratio of 5 : 1 without the addition of NaCl at pH 6.5. The Z-average diameter and zeta-potential values of RES-ALA-CHI nanoparticles were 211.0 nm and 13.23 mV, respectively. Both TEM and AFM graphs confirmed that RES-ALA-CHI nanoparticles had a spherical shape, and were dispersed homogeneously at the nanoscale. The encapsulation efficiency (EE) and loading amount (LA) of RES in RES-ALA-CHI nanoparticles were 58.86% and 196.2 μg mg-1, respectively, in the presence of 400 μg mL-1 RES. XRD results confirmed that RES was in amorphous form in ALA-CHI nanoparticles. The interaction between RES and ALA-CHI nanoparticles was mainly driven by hydrophobic interaction and hydrogen bonding. Compared to RES (free), the UV light and heat stability, in vitro bioaccessibility, and antioxidant activity of RES in RES-ALA-CHI nanoparticles were pronouncedly enhanced. The information provided in this study should be of interest to the food industry to fabricate robust nanoscale delivery systems with ALA-CHI nanoparticles for RES and other hydrophobic bioactive compounds.

中文翻译:

α-乳清蛋白和壳聚糖核-壳纳米颗粒:白藜芦醇的负载,保护和抗氧化活性。

负载白藜芦醇(RES)的蛋白质-多糖纳米颗粒是通过与带相反电荷的α-乳清蛋白(ALA)和壳聚糖(CHI)进行简单的静电相互作用制成的,质量比为5:1,而无需添加pH 6.5的NaCl。RES-ALA-CHI纳米粒子的Z平均直径和Zeta电位值分别为211.0 nm和13.23 mV。TEM和AFM图均证实RES-ALA-CHI纳米颗粒呈球形,并在纳米级均匀分散。在存在400μgmL-1 RES的条件下,RES-ALA-CHI纳米颗粒中RES的包封效率(EE)和负载量(LA)分别为58.86%和196.2μgmg-1。XRD结果证实RES在ALA-CHI纳米颗粒中为无定形形式。RES与ALA-CHI纳米粒子之间的相互作用主要是由疏水相互作用和氢键驱动的。与RES(不含)相比,RES-ALA-CHI纳米颗粒中RES的紫外光和热稳定性,体外生物可及性以及RES的抗氧化活性得到了显着提高。这项研究中提供的信息应为食品工业感兴趣,以使用用于RES和其他疏水性生物活性化合物的ALA-CHI纳米颗粒制造坚固的纳米级递送系统。
更新日期:2020-02-26
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