Viruses still pose a significant threat to human and animal health worldwide. In the fight against viral infections, high-purity viral stocks are needed for manufacture of safer vaccines. It is also a priority to ensure the viral safety of biopharmaceuticals such as blood products. Chromatography techniques are widely implemented at both academic and industrial levels in the purification of viral particles, whole viruses and virus-like particles to remove viral contaminants from biopharmaceutical products. This paper focuses on polysaccharide adsorbents, particulate resins and membrane adsorbers, used in virus purification/removal chromatography processes. Different chromatographic modes are surveyed, with particular attention to ion exchange and affinity/pseudo-affinity adsorbents among which commercially available agarose-based resins (Sepharose®) and cellulose-based membrane adsorbers (Sartobind®) occupy a dominant position. Mainly built on the development of new ligands coupled to conventional agarose/cellulose matrices, the development perspectives of polysaccharide-based chromatography media in this antiviral area are stressed in the conclusive part.

病毒仍然对全球人类和动物健康构成重大威胁。在抗击病毒感染中，需要高纯度的病毒储备来生产更安全的疫苗。确保诸如血液制品等生物药品的病毒安全性也是当务之急。色谱技术已在学术和工业水平上广泛用于纯化病毒颗粒，完整病毒和类病毒颗粒，以从生物制药产品中去除病毒污染物。本文重点研究用于病毒纯化/去除色谱过程的多糖吸附剂，颗粒树脂和膜吸附剂。调查了不同的色谱模式，特别注意离子交换和亲和/假亲和吸附剂，其中市售的琼脂糖基树脂（Sepharose®）和纤维素基膜吸附剂（Sartobind®）占据主导地位。主要在建立新配体与常规琼脂糖/纤维素基质偶联的基础上，在结语部分强调了基于多糖的层析介质在该抗病毒领域的发展前景。