Effective strategies for post-surgical adhesion prevention have increasingly focused on injectable adhesion barriers due to their minimal invasiveness and wider applicability. In this study, a thermo-reversible hydrogel was developed by combining high molecular weight hyaluronic acid (HA) at various concentrations (0.05, 0.25, and 0.45% w/v) with tempo-oxidized nanocellulose (TOCN), methyl cellulose (MC) and polyethylene glycol (PEG) for anti-adhesion application. The hydrogel preparation time was short and did not require any chemical modification. TOCN ensured the mechanical stability of the hydrogel. MC confirmed thermo-sensitive feature. Higher amounts of HA increased the rate of hydrogel degradation. The HA 0.25 hydrogel was free-flowing, injectable at ambient temperature, capable of faster (40 ± 2 s), and reversible sol-gel (4 °C–37 °C) transition. A rat side-wall cecum abrasion model was used to confirm the complete de novo adhesion prevention efficacy of optimized HA 0.25 hydrogel, where the scratched abdominal wall of animals treated with HA 0.25 hydrogel healed after 14 days. During in vivo experiment, PEG in the hydrogel played a crucial role in adhesion prevention by minimizing friction between the surgical site and nearby organs. In a nutshell, HA 0.25 hydrogel, fabricated without crosslinking agent, is a potential candidate for tissue adhesion prevention strategies.

预防手术后粘连的有效策略由于其最小的侵入性和更广泛的应用性，已越来越集中于可注射的粘连屏障。在这项研究中，通过组合各种浓度（0.05、0.25和0.45％w / v的高分子量透明质酸（HA），开发了一种热可逆水凝胶）和抗氧化的纳米纤维素（TOCN），甲基纤维素（MC）和聚乙二醇（PEG）。水凝胶的制备时间很短，不需要任何化学修饰。TOCN确保了水凝胶的机械稳定性。MC确认了热敏功能。高含量的HA增加了水凝胶的降解速率。HA 0.25水凝胶是自由流动的，可在环境温度下注射，能够更快（40±2 s），并且可逆的溶胶-凝胶（4°C–37°C）转变。使用大鼠侧壁盲肠磨损模型来确认优化的HA 0.25水凝胶的完全从头预防粘连功效，其中经HA 0.25水凝胶处理的动物的刮擦腹壁在14天后愈合。在体内实验中，水凝胶中的PEG通过最大程度地减少手术部位与附近器官之间的摩擦，在防止粘连中起着至关重要的作用。简而言之，无交联剂制备的HA 0.25水凝胶是预防组织粘连策略的潜在候选者。