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Expression analyses of C-type lectins (CTLs) in Manila clam under cold stress provide insights for its potential function in cold resistance of Ruditapes philippinarum.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.cbpc.2020.108708
Dongdong Li 1 , Hongtao Nie 1 , Kifat Jahan 1 , Xiwu Yan 1
Affiliation  

Manila clam Ruditapes philippinarum is an economically and scientifically important marine bivalve species. C-type lectin acts as a pattern-recognition receptor (PPR), plays a crucial role in the innate immunity of invertebrates, and involves in pathogen recognition, and cell-cell interaction. In this study, six different types of C-type lectin genes, CTL-1, CTL-2, CTL-3, CTL-4, CTL-5, and CTL-6, were identified from the R. philippinarum. CTL amino acid sequence was highly conserved compare to other invertebrate CTL sequences. Also, the temporal expressions of CTLs mRNA were detected in R. philippinarum with higher expression level in hepatopancreas and gill, and with lower expression levels in other tissues. The expression pattern of CTL genes in hepatopancreas were investigated under low-temperature stress using real-time quantitative fluorescence PCR (RT-qPCR) and the results showed that the transcription of CTL mRNAs were induced after low-temperature challenge. Overall, the sequence analysis and the expression patterns of CTLs provide clues for understanding the response of the Manila clam to low-temperature stress.

中文翻译:

马尼拉蛤中冷应激下C型凝集素(CTL)的表达分析为其在菲律宾蛤仔的耐寒性中的潜在功能提供了见识。

马尼拉蛤菲律宾蛤是经济上和科学上重要的海洋双壳类。C型凝集素起模式识别受体(PPR)的作用,在无脊椎动物的先天免疫中起关键作用,并参与病原体识别和细胞间相互作用。在这项研究中,从菲律宾蛤仔中鉴定出六种不同类型的C型凝集素基因CTL-1,CTL-2,CTL-3,CTL-4,CTL-5和CTL-6。与其他无脊椎动物CTL序列相比,CTL氨基酸序列高度保守。另外,在菲律宾蛤仔中检测到CTLs mRNA的时间表达,其在肝胰腺和g中的表达水平较高,而在其他组织中的表达水平较低。实时定量荧光PCR(RT-qPCR)研究了低温胁迫下肝胰腺CTL基因的表达模式,结果表明,低温刺激后CTL mRNA的转录被诱导。总体而言,CTL的序列分析和表达模式为了解马尼拉蛤对低温胁迫的响应提供了线索。
更新日期:2020-01-14
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