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Potential ameliorative effects of epigallocatechin-3-gallate against cigarette smoke exposure induced renal and hepatic deficits.
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.ecoenv.2020.110202
Jinglou Chen 1 , Jianhua Liu 1 , Yongfang Lei 2 , Min Liu 3
Affiliation  

The environmental pollution caused by cigarette smoke (CS) seriously endangers people's health. Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea. In this study, rats were exposed to CS for 90 days. Kidney function was evaluated by detecting the levels of serum creatinine and blood urea nitrogen. Liver function was evaluated by detecting the activities of alanine aminotransferase and aspartate transaminase. The renal and hepatic oxidative stress and inflammation were assessed by detecting the levels of malondialdehyde, reduced glutathione, antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and proinflammatory cytokines. Organ fibrosis was evaluated by observing collagen deposition via masson staining, by examining the hydroxyproline level, by measuring the mRNA levels of fibrosis-associated genes collagen (Col)-1A1 and Col-3A1, as well as by assessing the activity of profibrotic TGF-β1 pathway. Additionally, renal and hepatic epithelial-mesenchymal transition (EMT) were evaluated. It was observed that EGCG ameliorated the renal and hepatic oxidative stress, inflammation, EMT, as well as inhibited the activation of TGF-β1 signaling pathway induced by CS. These results showed that EGCG could attenuate CS-induced renal and hepatic fibrosis.

中文翻译:

表没食子儿茶素-3-没食子酸酯对香烟烟雾暴露引起的肾和肝功能损害的潜在改善作用。

香烟烟雾(CS)造成的环境污染严重危害人们的健康。Epigallocatechin-3-gallate(EGCG)是绿茶中含量最丰富的儿茶素。在这项研究中,大鼠暴露于CS 90天。通过检测血清肌酐和血尿素氮水平来评估肾脏功能。通过检测丙氨酸氨基转移酶和天冬氨酸转氨酶的活性来评估肝功能。通过检测丙二醛,还原型谷胱甘肽,抗氧化酶(超氧化物歧化酶和谷胱甘肽过氧化物酶)和促炎细胞因子的水平来评估肾脏和肝脏的氧化应激和炎症。通过观察通过Masson染色的胶原沉积,检查羟脯氨酸水平来评估器官纤维化,通过测量纤维化相关基因胶原(Col)-1A1和Col-3A1的mRNA水平,以及评估促纤维化TGF-β1途径的活性。另外,评估了肾和肝上皮-间质转化(EMT)。观察到EGCG改善了肾脏和肝脏的氧化应激,炎症,EMT,并抑制了CS诱导的TGF-β1信号通路的激活。这些结果表明,EGCG可以减轻CS诱导的肾和肝纤维化。并抑制CS诱导的TGF-β1信号通路的激活。这些结果表明,EGCG可以减轻CS诱导的肾和肝纤维化。并抑制CS诱导的TGF-β1信号通路的激活。这些结果表明,EGCG可以减轻CS诱导的肾和肝纤维化。
更新日期:2020-01-14
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