The severe and pervasive effects of multispecies foodborne microbial biofilms highlight the importance of rapid detection and diagnosis of contamination risk in the field using epifluorescence-based techniques (EBT) combined with automatic image-counting software. This study screened the hygiene quality of the environment, a carcass and the slaughtering equipment in the El-Kharga abattoir, New Valley Province, Egypt, to assess possible contamination during slaughter. In addition, the formed biofilm was assessed, and the bacteria were enumerated by epifluorescence microscopy. Using both conventional and EBT, the highest bacterial counts were observed for the slaughtering equipment (6.6 and 5.2 cfu/cm2, respectively), followed by different parts of the carcass (4.1 and 4.4 cfu/cm2, respectively) and environmental samples (3.9 and 4.1 cfu/cm2, respectively). A high prevalence of E. coli O157:H7 was observed on the slaughtering equipment (25%), which also led to carcass (1%) contamination. Moreover, Enterobacteriaceae members were detected during examination, such as Klebsiella pneumoniae, Enterobacter aerogenes, and Raoultella ornithinolytica. Despite the relatively good hygiene quality of the abattoir environment, there is also a high risk associated with biofilm formation by pathogenic microorganisms on the slaughtering equipment. Moreover, EBT showed different structures of the biofilm, including those formed at different maturation stages, such as voids, microbubbles, channels and mushroom shapes. (EBT) microscopy could be used to efficiently, precisely and rapidly estimate microbial aggregation and exposure risk inside the abattoir among conventional techniques.

多种食源性微生物生物膜的严重性和普遍性凸显了使用基于落射荧光的技术（EBT）结合自动图像计数软件来快速检测和诊断现场污染风险的重要性。这项研究筛选了埃及新谷省El-Kharga屠宰场的环境卫生质量，屠体和屠宰设备，以评估屠宰过程中可能的污染。另外，评估形成的生物膜，并通过落射荧光显微镜计数细菌。使用常规和EBT方法，屠宰设备的细菌计数最高（分别为6.6和5.2 cfu / cm2），其次是the体的不同部位（分别为4.1和4.4 cfu / cm2）和环境样品（分别为3.9和3.9 cfu / cm2）。 4.1 cfu / cm2，分别）。患病率很高在屠宰设备上观察到大肠杆菌O157：H7（占25％），这也导致car体污染（占1％）。此外，检查过程中检测到肠杆菌科成员，例如肺炎克雷伯氏菌，产气肠杆菌，和拉乌尔ornithinolytica。尽管屠宰场环境的卫生质量相对较好，但在屠宰设备上病原微生物形成生物膜也存在很高的风险。此外，EBT显示出不同的生物膜结构，包括在不同的成熟阶段形成的结构，例如空隙，微泡，通道和蘑菇形状。（EBT）显微镜可用于有效，准确和快速地评估传统技术中屠宰场内部的微生物聚集和暴露风险。