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DNA Amplifier-Functionalized Metal-Organic Frameworks for Multiplexed Detection and Imaging of Intracellular mRNA.
ACS Sensors ( IF 8.9 ) Pub Date : 2020-01-13 , DOI: 10.1021/acssensors.9b01759
Hong-Min Meng 1 , Xinxin Shi 1 , Juan Chen 1 , Yuhui Gao 1 , Lingbo Qu 1 , Ke Zhang 1 , Xiao-Bing Zhang 2 , Zhaohui Li 1
Affiliation  

DNA amplification is a useful technique for low-abundance biomarker detection and environmental monitoring because of its high signal-amplifying ability. However, intracellular application of DNA amplifiers remains challenging due to poor delivery efficiency and stability. Herein, we report an entropy-driven DNA amplifier-functionalized metal-organic framework (DNA amplifier-MOF) for the detection and imaging of multiple intracellular messenger RNAs (mRNAs). The DNA amplifier-MOF conjugate exhibits high cellular uptake, enhanced enzymatic stability, and good biocompatibility. Importantly, in the presence of phosphate ions, a surface-functionalized DNA amplifier can be released in cells with high efficiency, which facilitates the imaging of mRNA. This method is rapid and of high sensitivity/specificity, as validated in HepG2 and HL7702 cells for the imaging of TK1 and survivin mRNA, respectively. With further optimization, the strategy can become a powerful biotechnology tool for the detection of cancers at early stages and for elucidating biological processes.

中文翻译:

DNA扩增子功能化的金属有机框架,用于细胞内mRNA的多重检测和成像。

DNA扩增因其高信号放大能力而成为低丰度生物标志物检测和环境监测的有用技术。然而,由于差的递送效率和稳定性,DNA放大器的细胞内应用仍然具有挑战性。在这里,我们报告的熵驱动的DNA放大器功能化的金属有机框架(DNA放大器-MOF)用于检测和成像多个细胞内信使RNA(mRNA)。DNA放大器-MOF共轭物表现出高细胞摄取,增强的酶稳定性和良好的生物相容性。重要的是,在存在磷酸根离子的情况下,可以在细胞中高效释放表面功能化的DNA扩增子,从而促进mRNA成像。此方法快速且灵敏度高/专一,如分别在HepG2和HL7702细胞中验证的TK1和survivin mRNA成像一样。通过进一步优化,该策略可以成为强大的生物技术工具,用于早期检测癌症并阐明生物学过程。
更新日期:2020-01-14
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