The integration of sampling and instant metabolite readout can fundamentally elevate patient compliance. To circumvent the need for complex in-lab apparatus, here, an all-in-one sampling and display transdermal colorimetric microneedle patch was developed for sensing hyperglycemia in mice. The coloration of 3,3′,5,5′-tetramethylbenzidine (TMB) is triggered by the cascade enzymatic reactions of glucose oxidase (GOx) and horseradish peroxidase (HRP) at abnormally high glucose levels. The HRP in the upper layer is biomineralized with calcium phosphate (CaP) shell to add a pH responsive feature for increased sensitivity as well as protection from nonspecific reactions. This colorimetric sensor achieved minimally invasive extraction of the interstitial fluid from mice and converted glucose level to a visible color change promptly. Quantitative red green and blue (RGB) information could be obtained through a scanned image of the microneedle. This costless, portable colorimetric sensor could potentially detect daily glucose levels without blood drawing procedures.

采样和即时代谢物读数的集成可以从根本上提高患者的依从性。为了避免对复杂的实验室仪器的需求，在这里，开发了一种多合一采样和显示透皮比色微针贴片，用于感测小鼠的高血糖症。3,3'，5,5'-四甲基联苯胺（TMB）的着色是由葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）在异常高葡萄糖水平下的级联酶促反应触发的。上层的HRP用磷酸钙（CaP）外壳进行生物矿化处理，以增加pH响应特性，从而提高灵敏度并防止非特异性反应。该比色传感器实现了从小鼠中微创抽取组织液，并迅速将葡萄糖水平转换为可见的颜色变化。通过微针的扫描图像可以获得定量的红色，绿色和蓝色（RGB）信息。这种无需花费的便携式比色传感器可以潜在地检测每日的血糖水平，而无需进行抽血程序。