The sensitivity for singlet oxygen (1O2) of two convenient 1O2 probes, 1,3-diphenylisobenzofuran (DPBF) and 9,10-Anthracenediyl-bis(methylene)dimalonic acid (ABDA), has been investigated in different aqueous environments. Both probes are commercially available at reasonable cost and can be used with standard UV-vis spectrometers. Although DPBF is not soluble in neat water and is not specific to the detection of 1O2, it has very high, essentially diffusion-limited, reactivity towards 1O2; it can trap up to 50% of all 1O2 created in alcohol/water or micellar solution, and even more when replacing H2O by D2O, which makes it highly useful when the process under investigation does not yield much 1O2. On the other hand, ABDA has a much lower reactivity, reacting with only 2% of the singlet oxygen generated in H2O, as well as a smaller extinction coefficient, resulting in a much smaller spectroscopic response, but is soluble in neat water and is specific for 1O2, allowing for discrimination from other reactive oxygen species. The results presented here not only allow a comparative assessment of the usefulness of the two 1O2 probes, but also provide a reference for an accurate absolute quantification of the amount of 1O2 generated in an experiment from the observed absorbance bleach.

中文翻译：

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水性环境中常用的单线态氧探针的检测灵敏度。

在不同的水环境中，研究了两种方便的1O2探针1,3-二苯基异苯并呋喃（DPBF）和9,10-蒽二基-双（亚甲基）二氢膦酸（ABDA）对单线态氧（1O2）的敏感性。两种探针都可以以合理的价格在市场上买到，并且可以与标准的紫外可见光谱仪一起使用。尽管DPBF不溶于纯净水，并且不是特定于1O2的检测，但它对1O2的反应性非常高，基本上是受扩散限制的。它可以捕集高达50％的乙醇/水或胶束溶液中产生的1O2，当用D2O代替H2O时可以捕集更多，这在所研究的过程中不会产生太多1O2时非常有用。另一方面，ABDA的反应性要低得多，只与H2O中产生的2％单线态氧发生反应，以及较小的消光系数，从而导致光谱响应小得多，但可溶于纯净水，并且对1O2具有特异性，因此可以区别于其他活性氧。此处提供的结果不仅可以对两种1O2探针的有用性进行比较评估，而且可以为从实验中观察到的吸光度漂白剂准确地绝对定量实验中产生的1O2量提供参考。