Abstract A novel fluorescent nanometer biosensor integrating a β-cyclodextrin-modified carbon dot (β-CD-CD) fluorescent nanoprobe and ferrocene-marked adapter (ssDNA-Fc) was developed and used to measure platelet-derived growth factor (PDGF)-BB. When no PDGF-BB was added, ssDNA-Fc induced the enhanced fluorescence of β-CD-CD nanoprobes. After adding PDGF-BB, ssDNA-Fc specifically bonded with PDGF-BB. Meanwhile, the β-CD-CDs and Fc in ssDNA-Fc initiated host–guest identification, which then induced photoinduced electron transfer and decreased fluorescence, thereby contributing to the high-sensitivity monitoring of PDGF-BB. This novel approach showed a wide linear range of 10 pg mL−1 to 8 μg mL−1 and a low detection limit of 6 pg mL−1 (RSD = ±4.6%). Moreover, the spiked recovery rate in human serum was 98.2%–106.2% (RSD ≤ ±3.79%), indicating that this method can be used to monitor PDGF-BB in human serum.

中文翻译：

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基于二茂铁标记适配器的灵敏生物传感器，用于血小板衍生生长因子 BB 的荧光检测

摘要 开发了一种集成 β-环糊精修饰碳点 (β-CD-CD) 荧光纳米探针和二茂铁标记接头 (ssDNA-Fc) 的新型荧光纳米生物传感器，用于测量血小板衍生生长因子 (PDGF)-BB . 当不添加 PDGF-BB 时，ssDNA-Fc 会诱导 β-CD-CD 纳米探针的荧光增强。添加 PDGF-BB 后，ssDNA-Fc 与 PDGF-BB 特异性结合。同时，ssDNA-Fc中的β-CD-CDs和Fc启动了宿主-客体识别，然后诱导光诱导电子转移和荧光减弱，从而有助于PDGF-BB的高灵敏度监测。这种新颖的方法显示出 10 pg mL-1 至 8 μg mL-1 的宽线性范围和 6 pg mL-1 的低检测限（RSD = ±4.6%）。此外，人血清中的加标回收率为 98.2%–106.2% (RSD ≤ ±3.79%)，