Minocycline (MINO) is a tetracycline antibiotic effective against most of the bacteria microorganisms related to periodontal disease. Additionally, MINO promotes bone in vitro and in vivo. The objective of the present study was to establish the protocol for the preparation of MINO-loaded poly (lactic-co-glycolic acid) (MINO-PLGA) electrospun membranes and to evaluate their effect on osteogenesis in vitro and in a rat model of periodontitis. The characterization of MINO-PLGA electrospun membranes was assessed by scanning electron microscopy, laser scanning confocal microscopy, and contact angle measurement. The drug release study showed a sustained diffusion of MINO from electrospun membranes over a period of 40 d. The MINO-PLGA membranes containing 2% of the drug exhibited better support of osteoblast proliferation and adhesion and was subsequently used in vivo in an experimental periodontitis model. Its therapeutic potential was evaluated by the measurement of alveolar bone loss (ABL), bone volume analysis, histological analysis, and immunohistochemistry. MINO-PLGA membrane increased alveolar crest height in the periodontitis model, inhibited the expression of the ligand of the receptor activator for nuclear factor-κB (RANKL), and promoted the expression of its inhibitor, osteoprotegerin. The study demonstrated that MINO-PLGA electrospun membranes may be applied to stimulate bone regeneration in periodontitis.

中文翻译：

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载有米诺环素的PLGA电纺膜可防止实验性牙周炎引起的牙槽骨丢失。

Minocycline（MINO）是一种四环素抗生素，对与牙周疾病有关的大多数细菌微生物有效。此外，MINO可以在体内和体外促进骨骼。本研究的目的是建立制备载有MINO的聚乳酸-乙醇酸（MINO-PLGA）电纺膜的方案，并评估其在体外和大鼠牙周炎模型中对成骨的影响。通过扫描电子显微镜，激光扫描共聚焦显微镜和接触角测量来评估MINO-PLGA电纺膜的特性。药物释放研究表明，在40天内，MINO从电纺膜中持续扩散。含有2％药物的MINO-PLGA膜表现出对成骨细胞增殖和粘附的更好支持，随后被用于体内实验性牙周炎模型。通过测量牙槽骨丢失（ABL），骨体积分析，组织学分析和免疫组织化学来评估其治疗潜力。在牙周炎模型中，MINO-PLGA膜可增加牙槽rest高度，抑制核因子-κB受体激活剂（RANKL）的配体表达，并促进其抑制剂骨保护素的表达。研究表明，MINO-PLGA电纺膜可用于刺激牙周炎的骨再生。骨体积分析，组织学分析和免疫组织化学。在牙周炎模型中，MINO-PLGA膜可增加牙槽c高度，抑制核因子-κB受体激活剂（RANKL）的配体表达，并促进其抑制剂骨保护素的表达。研究表明，MINO-PLGA电纺膜可用于刺激牙周炎的骨再生。骨体积分析，组织学分析和免疫组织化学。在牙周炎模型中，MINO-PLGA膜可增加牙槽c高度，抑制核因子-κB受体激活剂（RANKL）的配体表达，并促进其抑制剂骨保护素的表达。研究表明，MINO-PLGA电纺膜可用于刺激牙周炎的骨再生。