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Relative potency factor approach enables the use of in vitro information for estimation of human effect factors for nanoparticle toxicity in life-cycle impact assessment.
Nanotoxicology ( IF 5 ) Pub Date : 2020-01-13 , DOI: 10.1080/17435390.2019.1710872
Beatrice Salieri 1 , Jean-Pierre Kaiser 2 , Matthias Rösslein 2 , Bernd Nowack 1 , Roland Hischier 1 , Peter Wick 2
Affiliation  

The major theme of the NRC report "Toxicity Testing in the Twenty-first Century" is to replace animal testing by using alternative in vitro methods. Therefore, it can be expected that in the future in vivo data will be replaced with in vitro data. Hence, there is a need for new strategies to make use of the increasing amount of in vitro data when developing human toxicological effect factors (HEF) to characterize the impact category of human toxicity in life cycle assessment (LCA). Here, we present a new approach for deriving HEF for manufactured nanomaterials (MNMs) based on the combined use of in vitro toxicity data and a relative potency factor (RPF) approach. In vitro toxicity tests with nano-CuO, nano-Ag and nano-ZnO and their corresponding ions were performed on THP-1, CaCo-2 and Hep-G2 cell lines. The ratio of the here calculated EC50 of the ionic form and the nanoform corresponds to the Relative Potency Factor (RPF). Using this approach, HEFs (case/kgintake) for the aforementioned nanoparticles were obtained. Non-carcinogenic HEFs (case/kgintake) for exposure via ingestion of 5.9E-01, 7.5E-03 and 2.5 E-02 were calculated for nano-Ag, nano-CuO and nano-ZnO, respectively. The HEF values here proposed were compared with HEF values extrapolated from in vivo toxicity data reported in the literature. The here presented procedure is the most appropriate approximation currently available for using in vitro toxicity data on MNM for application in the field of LCIA.

中文翻译:

相对效能因子方法可用于在生命周期影响评估中使用体外信息来评估人类影响因子对纳米颗粒毒性的影响。

NRC报告“二十一世纪的毒性测试”的主题是通过使用替代的体外方法代替动物测试。因此,可以预期,将来体内数据将被体外数据取代。因此,在开发人类毒理学影响因子(HEF)来表征生命周期评估(LCA)中人类毒性的影响类别时,需要一种新的策略来利用越来越多的体外数据。在这里,我们结合体外毒性数据和相对效能因子(RPF)方法,提出了一种新的方法来推导人造纳米材料(MNM)的HEF。在THP-1,CaCo-2和Hep-G2细胞系上进行了纳米CuO,纳米Ag和纳米ZnO及其相应离子的体外毒性测试。此处计算的离子形式和纳米形式的EC50之比对应于相对效能因子(RPF)。使用这种方法,获得了用于上述纳米颗粒的HEF(案例/千克摄入量)。通过摄入5.9E-01、7.5E-03和2.5 E-02分别计算了纳米Ag,纳米CuO和纳米ZnO的非致癌性HEF(病例/千克摄入量)。将此处提出的HEF值与从文献中报道的体内毒性数据推断出的HEF值进行了比较。此处介绍的过程是当前可用于在LCIA领域中使用MNM上的体外毒性数据的最合适的近似方法。通过摄入5.9E-01、7.5E-03和2.5 E-02分别计算了纳米Ag,纳米CuO和纳米ZnO的非致癌性HEF(病例/千克摄入量)。将此处提出的HEF值与从文献中报道的体内毒性数据推断出的HEF值进行了比较。此处介绍的过程是当前可用于在LCIA领域中使用MNM上的体外毒性数据的最合适的近似方法。通过摄入5.9E-01、7.5E-03和2.5 E-02分别计算了纳米Ag,纳米CuO和纳米ZnO的非致癌性HEF(病例/千克摄入量)。将此处提出的HEF值与从文献中报道的体内毒性数据推断出的HEF值进行了比较。此处介绍的过程是当前可用于在LCIA领域使用MNM上的体外毒性数据的最合适的近似方法。
更新日期:2020-01-13
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