A novel visual detection method was developed for H₂O₂ and glucose detection via the _L-Cysteine (_L-Cys) mediated synthesis of quantum dots (QDs). The mechanism of the ligand effect during QDs synthesis was also studied by theoretical calculation. This strategy is based on that _L-Cys can be used as both H₂O₂ substrate and ligand to mediate biomimetic synthesis of luminescent CdTe QDs. Iodide (I-) can catalytic oxidized _L-Cys to _L-cystine in the presence of H₂O₂, which inhibits the synthesis of QDs. Hence, the degree of inhibition for the QDs synthesis is proportional to the H₂O₂ concentration. Additionally, the ligand effect of _L-Cys and _L-cystine for CdTe QDs synthesis was studied. The density functional theory (DFT) calculations showed that the combination of CdTe QDs with _L-Cys had higher energy and more stable configuration than that with _L-cystine, indicating that _L-Cys is the more stable ligand of QDs. Under the optimized conditions, a low limit of detection (LOD) was obtained for H₂O₂ (0.3 nM). Then, we used H₂O₂ as a bridge for glucose detection, with a LOD of 5 nM. The feasibility of this sensor was demonstrated by rapid detection of H₂O₂ and glucose by naked-eye at a level of 10 nM.

一种新的视觉检测方法用于h开发₂ ö ₂经由和葡萄糖检测_大号半胱氨酸（_大号-Cys）量子点（QD）的介导的合成。还通过理论计算研究了量子点合成过程中配体效应的机理。该策略基于_L -Cys既可以用作H ₂ O ₂底物又可以用作配体来介导发光CdTe QD的仿生合成。碘化物（I-）在H ₂ O ₂存在下可将氧化的_L -Cys催化氧化为_L-胱氨酸，这会抑制QD的合成。因此，对QDs合成的抑制程度与H ₂ O ₂浓度成正比。另外，研究了_L -Cys和_L-胱氨酸对CdTe QDs合成的配体作用。密度泛函理论（DFT）计算表明，与_L-胱氨酸相比，CdTe QD与_L -Cys的结合具有更高的能量和更稳定的构型，表明_L -Cys是QDs的更稳定的配体。在优化的条件下，获得了H ₂ O ₂（0.3 nM）的低检测限（LOD ）。然后，我们使用了H ₂ O ₂作为检测葡萄糖的桥梁，LOD为5 nM。该传感器的可行性通过肉眼以10 nM的水平快速检测H ₂ O ₂和葡萄糖来证明。