In this work, a novel high electro-catalytic material was synthesized using PtNi nanospheres to functionalize two-dimensional (2D) ultrathin Cu-TCPP(Fe) nanosheets ([email protected](Fe)), and was further applied to develop an enzyme-free electrochemical immunosensor for the ultrasensitive determination of calprotectin (CALP). The bimetallic Cu-TCPP(Fe) nanosheets with large surface area and massive accessible active sites permitted multiple PtNi to attach their surface, which not only enhanced the catalytic ability and conductivity for non-enzymatic amplification but also provided the modified active sites for antibody immobilization as signal labels. Upon the dual electro-catalytic activity of Cu-TCPP(Fe) and PtNi towards H₂O₂ reduction for signal amplification, this proposed sandwich CALP immunosensor exhibited a wide linear range of 200 fg mL^-1 ∼ 50 ng mL^-1 and a low detection limit of 137.7 fg mL^-1. The functionalized MOF nanosheets-based biosensing method offered an enzyme-free signal amplification strategy, which provided great promise for further bioanalysis and clinical study.

在这项工作中，使用PtNi纳米球合成了一种新型的高电催化材料，以对二维（2D）超薄Cu-TCPP（Fe）纳米片（[电子邮件保护]（Fe））进行功能化，并进一步用于开发酶。的电化学免疫传感器用于钙卫蛋白（CALP）的超灵敏测定。具有大表面积和可访问的大量活性位点的双金属Cu-TCPP（Fe）纳米片允许多个PtNi附着其表面，这不仅增强了非酶促扩增的催化能力和电导率，而且还为抗体固定化提供了修饰的活性位点作为信号标签。Cu-TCPP（Fe）和PtNi对H ₂ O ₂的双重电催化活性为了减少信号放大，该拟议的夹心CALP免疫传感器具有200 fg mL ^-1到50 ng mL ^-1的宽线性范围和137.7 fg mL ^-1的低检测限。基于功能化MOF纳米片的生物传感方法提供了无酶信号放大策略，这为进一步的生物分析和临床研究提供了广阔的前景。