In this study we have reported the design and development of a facile, sensitive, selective, and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers. Electrospun nanofibers were characterized using scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscope (XPS), UV-Visible spectroscope (UV-VIS), and Fourier-transform infrared spectroscope (FTIR). Electrochemical properties of MWCNT-ZnO nanofiber-modified electrodes were assessed using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Binding event of atrazine to anti-atrazine antibody, which immobilized on nanofiber-modified electrode via EDC and NHS chemistry, was transduced with EIS. Due to high conductivity, surface area, and low bandgap of MWCNT-ZnO nanofibers, we have achieved the sensitivity and limit of detection (LoD) of sensor as 21.61 (KΩ μg⁻¹ mL⁻¹) cm⁻² and 5.368 zM for a wide detection range of 10 zM–1 µM. The proposed immunosensing platform has good stability, selectivity, repeatability, and reproducibility, and are less prone to interference.

在这项研究中，我们报道了一种基于 MWCNT 嵌入的 ZnO 纳米纤维的用于检测莠去津的简便、灵敏、选择性和无标记的电化学传感平台的设计和开发。电纺纳米纤维使用扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、X 射线衍射 (XRD)、X 射线光电子能谱仪 (XPS)、紫外-可见分光镜 (UV-VIS) 和傅立叶变换进行表征红外光谱仪 (FTIR)。使用电化学阻抗谱 (EIS) 和循环伏安法 (CV) 评估 MWCNT-ZnO 纳米纤维改性电极的电化学性能。阿特拉津与抗阿特拉津抗体的结合事件，通过 EDC 和 NHS 化学固定在纳米纤维修饰的电极上，用 EIS 转导。由于电导率高，表面积大，^-1  mL ^-1 ) cm ^-2和 5.368 zM，用于 10 zM–1 µM 的宽检测范围。所提出的免疫传感平台具有良好的稳定性、选择性、重复性和再现性，并且不易受到干扰。